利用静电纺丝技术制备了再生丝素蛋白纤维毡,并采用乙醇水溶液对其进行拉伸后处理以提高其力学性能。研究了乙醇的体积分数对纤维毡形貌、结构和性能的影响。结果表明,随乙醇体积分数的增加,纤维毡中β-折叠结构的含量增加。纤维毡在体积分数为90%的乙醇溶液中,先拉伸1.11倍,然后浸泡120min后,其断裂强度及断裂伸长率分别达2.64MPa和12.1%。
Regenerated silk fibroin mats were prepared using electrospinning method and post-drawn in ethanol aqueous solution to improve mechanical properties.The effects of ethanol volume fraction of the solution on the morphology,secondary structure and properties of the mats were investigated.It was found that with the increase of the ethanol volume fraction,the β-sheet content of the mats increased.The mats,which were post-drawn 1.11 times and then immersed 120min in 90vol% ethanol,achieved a breaking strength of 2.64MPa and a breaking elongation of 12.1%.
参考文献
| [1] | Zhang Xiaohui;Reagan Michaela R;Kaplan David L .Electrospun silk biomateriat scaffolds for regenerativemedicine[J].Advanced Drug Delivery Reviews,2009,61(12):988-1006. |
| [2] | 胡雯,黄争鸣,唐亮.PLLA纳米纤维编织缝合线的制备及生物相容性研究[J].功能材料,2009(11):1888-1891,1899. |
| [3] | Zhang XH;Baughman CB;Kaplan DL .In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth[J].Biomaterials,2008(14):2217-2227. |
| [4] | Wang Y;Rudym DD;Walsh A;Abrahamsen L;Kim HJ;Kim HS;Kirker-Head C;Kaplan DL .In vivo degradation of three-dimensional silk fibroin scaffolds.[J].Biomaterials,2008(24/25):3415-3428. |
| [5] | Altman GH;Horan RL;Lu HH;Moreau J;Martin I;Richmond JC;Kaplan DL .Silk matrix for tissue engineered anterior cruciate ligaments.[J].Biomaterials,2002(20):4131-4141. |
| [6] | Horan RL;Antle K;Collette AL;Wang Y;Huang J;Moreau JE;Volloch V;Kaplan DL;Altman GH .In vitro degradation of silk fibroin.[J].Biomaterials,2005(17):3385-3393. |
| [7] | Reneker Darrell H;Chun Iksoo .Nanometre diameter fibres of polymer,produced by electrospinning[J].Nanotechnology,1996,7(03):216-223. |
| [8] | 杭怡春,张耀鹏,邵惠丽,胡学超.再生丝素/丝胶共混蛋白水溶液的静电纺丝[J].功能材料,2010(01):108-111. |
| [9] | Jin HJ;Chen J;Karageorgiou V;Altman GH;Kaplan DL .Human bone marrow stromal cell responses on electrospun silk fibroin mats.[J].Biomaterials,2004(6):1039-1047. |
| [10] | Meinel Anne J;Kubow Kristopher E;Klotzsch Enrico et al.Optimization strategies for eleetrospun silk fibroin tissue engineering scaffolds[J].Biomaterials,2009,30(17):3058-3067. |
| [11] | Hui Cao;Xin Chen;Lei Huang;Zhengzhong Shao .Electrospinning of reconstituted silk fiber from aqueous silk fibroin solution[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2009(7):2270-2274. |
| [12] | Tanabe Yoshikazu;Kanetsuna Hisaaki .Structure of oriented polystyrene monofilaments and its relationship to brittle-to- ductile transition[J].Journal of Applied Polymer Science,1978,22(06):1619-1630. |
| [13] | Guanqiang Zhou;Zhengzhong Shao;David P. Knight;Jiaping Yan;Xin Chen .Silk Fibers Extruded Artificially From Aqueous Solutions Of Regenerated Bombyx Mori Silk Fibroin Are Tougher Than Their Natural Counterparts[J].Advanced Materials,2009(3):366-370. |
| [14] | Wei Wei;Yaopeng Zhang;Huili Shao;Xuechao Hu .Posttreatment of the dry-spun fibers obtained from regenerated silk fibroin aqueous solution in ethanol aqueous solution[J].Journal of Materials Research,2011(9):1100-1106. |
| [15] | Wei Wei;Yaopeng Zhang;Yingmei Zhao;Huili Shao;Xuechao Hu .Studies on the post-treatment of the dry-spun fibers from regenerated silk fibroin solution; Post-treatment agent and method[J].Materials & design,2012(Apr.):816-822. |
| [16] | Monti P;Freddi G;Bertoluzza A et al.Raman spectroseopic studies of silk fibroin from Bombyx mori[J].Journal of Raman Spectroscopy,1998,29(04):297-304. |
| [17] | Ping Z;Xun X;Knight DP;Zong XH;Feng D;Yao WH .Effects of pH and calcium ions on the conformational transitions in silk fibroin using 2D Raman correlation spectroscopy and C-13 solid-state NMR[J].Biochemistry,2004(35):11302-11311. |
| [18] | Asakura Tetsuo;Yao Juming;Yamane Tsutomu et al.Heterogeneous structure of silk fibers from Bombyx mori resolved by C-13 solid-state NMR spectroscopy[J].Journal of the American Chemical Society,2002,124(30):8794-8795. |
| [19] | Zhou L;Chen X;Shao ZZ;Huang YF;Knight DP .Effect of metallic ions on silk formation the mulberry silkworm, Bombyx mori[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2005(35):16937-16945. |
| [20] | Ha Sung Won;Park Young H;Hudson Samuel M .Dissolution of bombyx mori silk fibroin in the calcium nitrate tetrahydrate-methanol system and aspects of wet spinning of fibroin solution[J].Biomacromolecules,2003,4(03):488-496. |
| [21] | 陈新,周丽,邵正中,周平,KNIGHT David P,VOLLRATH Fritz.时间分辨红外光谱对丝蛋白膜构象转变动力学的研究--再生蚕丝蛋白膜在高浓度醇溶液中的构象转变[J].化学学报,2003(04):625-629. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%