本文以牛骨粉为原料,采用酶解法制备明胶,考察了骨粉预处理试剂——盐酸的浓度对明胶产品的冻力、粘度、分子量分布和等电点的影响.研究表明,随着骨粉预处理用的盐酸浓度的增大,明胶产率降低.骨粉经过盐酸预处理后,明胶产品的冻力大于300 bloom g,高于原料未经盐酸处理得到的明胶产品.与传统的碱法工艺明胶相比,酶法明胶具有高冻力、高等电点和相对较低的粘度等特点.采用聚丙烯酰胺凝胶电泳表征明胶的分子质量分布,酶法明胶不但具有传统碱法明胶具有的96 KDa、97 KDa、193 KDa和289 KDa组分,而且具有165 KDa和120 KDa组分.酶法明胶的等电点在碱性范围内,随着预处理用盐酸浓度的增大,明胶产品的等电点升高.骨料的预处理工艺对明胶的产率、冻力、粘度、等电点和分子质量分布有重要的影响,在明胶制备过程中,通过调控骨料预处理酸浓度可以调控明胶的技术指标.
参考文献
| [1] | Karim A;Rajeev B .Fish gelatin:properties,challenges,and prospects as an alternative to mammalian gelatins[J].Food Hydrocolloids,2009,23:567-576. |
| [2] | Olsen D;Yang C;Bodo M;Chang R;Leigh S;Baez J;Carmichael D;Perala M;Hamalainen ER;Jarvinen M;Polarek J .Recombinant collagen and gelatin for drug delivery.[J].Advanced drug delivery reviews,2003(12):1547-1567. |
| [3] | Reinhard S;Herbert G.Gelatin Handbook:Theory and Industrial Practice[M].Weinheim:WILEY-VCH Press,2007:1-36. |
| [4] | 缪进康.明胶及其在科技领域中的利用[J].明胶科学与技术,2009(01):28-49,51. |
| [5] | 姚龙坤,顾平远,黄文涛.明胶在食品工业中的应用简述[J].明胶科学与技术,2009(01):24-25. |
| [6] | Farah Badii;Nazlin K. Howell .Fish gelatin: Structure, gelling properties and interaction with egg albumen proteins[J].Food hydrocolloids,2006(5):630-640. |
| [7] | Zhang F;Xu S;Wang Z.Pre-treatment optimization and properties of gelatin from freshwater fish scales[J].Food and Bioproducts Processing,2010 |
| [8] | Chomarat N;Robert L;Seris J et al.Comparative efficiency of pepsin and proctase for the preparation of bovine skin gelatin[J].Enzyme and Microbial Technology,1994,16(09):756-760. |
| [9] | Cannan R;Muntwyler E .The action of pepsin on gelatin[J].Biochemical Journal,1930,24(04):1012-1020. |
| [10] | 陈丽娟 等.酶降解骨胶原制备明胶的方法[P].中国专利,ZL02 125 447.8,2005-4-13. |
| [11] | 张锋,施辉阳,张鹏.酶法制备明胶的新工艺研究[J].北京化工大学学报(自然科学版),2003(01):9-12. |
| [12] | 陈丽娟,王颖,彭必先.胰蛋白酶降解骨胶原与明胶组份含量之间的关系[J].感光科学与光化学,1998(03):258. |
| [13] | Nadia Elharfaoui;Madeleine Djabourov;Wilfried Babel .Molecular Weight Influence on Gelatin Gels: Structure, Enthalpy and Rheology[J].Macromolecular symposia,2007(0):149-157. |
| [14] | M.H. Norziah;A. Al-Hassan;A.B. Khairulnizam;M.N. Mordi;M. Norita .Characterization of fish gelatin from surimi processing wastes: Thermal analysis and effect of transglutaminase on gel properties[J].Food hydrocolloids,2009(6):1610-1616. |
| [15] | Mechanical properties of mammalian and fish gelatins based on their weight average molecular weight and molecular weight distribution[J].Food hydrocolloids,2009(8):2315. |
| [16] | Akkasit Jongjareonrak;Saroat Rawdkuen;Manat Chaijan;Soottawat Benjakul;Kazufumi Osako;Munehiko Tanaka .Chemical compositions and characterisation of skin gelatin from farmed giant catfish (Pangasianodon gigas).[J].LWT-Food Science & Technology,2010(1):161-165. |
| [17] | EastoeJ;Leach A.Chemical constitution of gelatin[A].London,UK:Academic Press,1977 |
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