将壳聚糖微球/磷酸钙骨水泥复合材料植入兔股骨髁内,进行大体观察和X光片观察,并分别在植入兔股骨髁内8、16和24周,取出样本进行组织学观察和环境扫描电镜观察,研究植入材料与骨组织界面的结合状况和材料在动物体内的降解过程及新骨重建生成状况,探索材料在体内的降解机制和成骨机制.结果表明:壳聚糖微球/磷酸钙骨水泥在骨组织内具有良好的生物相容、组织相容性和和骨结合性,能够与自然骨组织形成紧密的骨性结合.材料在体内具有较快的降解速度,植入24周后,80%以上材料降解,被新生骨组织替代,该材料还具有良好的可塑性和临床操作性,是一种很有临床应用前景的骨修复材料.
The in vivo behavior study was performed to evaluate the osteogenous ability and biocompatibility of Composite of Chitosan Microspheres/Calcium Phosphate Cement. Twelve four-week-old New Zealand white rabbits were divided into four groups randomly, three in each group. According to the principle of self-contrast at the same time, cavities of 5 mm in diameter and 6 mm in depth were drilled in femoral condyle of rabbits. The materials were implanted into cavities of the left leg, and the right leg as the blank control group. Rabbits were killed at 4, 8, 16 and 24 weeks after surgery. The microstructure of specimens was observed using ESEM. The results show that the Composite of Chitosan Microspheres/Calcium Phosphate Cement has good fluidity and plasticity; it can be injected into bone defects and fast-set in situ. The start setting time is 5～15 min and the compressive strength is 15～20 MPa. The Composite of Chitosan Microspheres/Calcium Phosphate Cement has good biocompatibility and osteoconductivity, and benefits to the repair of bone defects.
参考文献
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