制备了一种新型应用于生物芯片的导电聚合物敏感膜,研究了蛋白质在导电聚合物敏感膜表面上的吸附机理.首 先合成巯基修饰的吡咯单体,然后通过自组装在金膜表面制备N-巯基吡咯自组装膜,并通过化学法制备聚合吡咯膜,详细考察了牛血清白蛋白(BSA)在敏感膜上的吸附行为.用傅立叶红外光谱(FT-IR)和X射线光电子能谱(XPS)考察了自组装膜及聚合吡咯膜的化学结构.用表面等离子谐振仪考察了BSA在自组装膜和聚合吡咯膜上的吸附行为,以及聚合时间、缓冲液pH值对BSA吸附的影响.
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