基于在反应体系中添加碳酸氢铵可有效地促进酶促外消旋对羟基苯甘氨酸甲酯不对称水解这一有趣现象的发现,探讨了利用有机介质中酶促外消旋对羟基苯甘氨酸甲酯不对称水解制备对映体纯D-对羟基苯甘氨酸及其衍生物的可能性,研究了不同来源的酶、摇床转速、水含量、对羟基苯甘氨酸甲酯浓度、反应介质及温度等因素对该反应的影响. 结果表明,在所研究的11种酶中脂肪酶Novozym 435对该反应的催化活性和对映体选择性较高,叔丁醇为最合适的反应介质,最适水含量为0.4%(V/V),对羟基苯甘氨酸甲酯的适宜浓度为20 mmol/L,最适摇床转速和反应温度分别为130 r/min和30 ℃. 在此优化条件下反应26 h后,底物转化率和产物ee分别为39.8%和95.2%.
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