观察Ti-Nb-Zr-Sn系钛合金两种表面形态对兔腱膜成纤维细胞生物学行为的影响.评价其生物相容性.采用组织块法培养原代兔跟腱腱膜成纤维细胞并传代后,接种至光滑表面和规则微纹理表面两组钛合金薄片上,通过MTT比色法、扫描电镜等技术,观察细胞在不同微形态钛合金表面的增殖和贴附状况.兔腱膜成纤维细胞在两种形态钛材料表面上的细胞增殖速率不同,其增殖速率的吸光度峰值在光滑组为0.228,微纹理组为0.316.有明显统计学差异(P<0.01).扫描电镜下观察,光滑组成纤维细胞扁平,伪足少,贴附无定向性;规则微纹理组的细胞胞体饱满、伪足数量较多且相互连接,沿材料表面纹理贴附,其长轴与纹理方向一致.Ti-Nb-Zr-Sn系钛合金的规则微纹理表面处理有利于动物成纤维细胞的附着,其生物相容性优于光滑表面.
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