利用锂钙硼玻璃在磷酸盐溶液中的原位转化反应制备表面多孔且具有中空层状结构的羟基磷灰石（HA）微球,以溶菌酶为蛋白的药物模型,研究了中空层状结构的羟基磷灰石微球对溶菌酶的吸附及缓释特性,结果显示,中空微球对不同浓度的溶菌酶溶液,具有不同的吸附机理,当溶菌酶溶液的浓度低于0.8mg/mL时,溶菌酶的吸附主要发生在微球的外表面,符合Langmuir模型,释放速率较快,48h内基本释放完全;当溶菌酶溶液的浓度高于0.8mg/mL时,溶菌酶扩散进入微球内部及球壁的微孔中,使得吸附量显著增加,满足Henry吸附模型,溶菌酶的释放周期明显增加,可持续释放800h,微球对蛋白具有很好的缓释效果。
Hollow hydroxyapatite microspheres,consisting of a hollow core and a multilayer porous shell,were prepared by converting Li2O-CaO-B2O3 glass microspheres in dilute phosphate solution.Lysozyme was used as the drug model of proteins,then the adsorption and in vitro drug release behaviors were studied.It was found that the adsorption mechanism was different with various concentration of lysozyme solution.When the concentration was lower than 0.8mg/mL,the adsorption was occurred on the surface of microspheres,the adsorption behavior was in accordance with the Langmuir adsorption model.In vitro release showed lysozyme was released completely in 48h.When the concentration was higher than 0.8mg/mL,more adsorption of lysozyme was occurred on the inner shell of the microsphere,the adsorption behavior was in accordance with the Henry adsorption model.And the release of lysozyme from the hollow HA microspheres could be lasted an extended period（up to 800h）.The present delivery system established could be used as the controlled drug release carrier for proteins,such as growth factor.
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