静电纺丝法制备的聚（D,L-乳酸）超细纤维具有孔隙率高、孔径/结构可调、生物相容性良好等特点,已成为组织工程支架材料研究的热点。探讨了纺丝溶液浓度、流量、电压、干燥方式等影响聚（D,L-乳酸）纤维支架形貌的因素,确定了最佳工艺参数为纺丝溶液浓度为0.20g/mL,流速为0.8mL/h,电压为12kV,经真空冷冻干燥,可获得分布比较均匀,直径大多为500～900nm的聚乳酸纤维。体外细胞形貌观察表明聚（D,L-乳酸）纤维支架具有良好的细胞相容性。
Electrospun poly（D,L,lactic acid）（PDLLA） superfine fiber has become one of the hot spots regarding scaffolds materials in tissue engineering,due to its high porosity,tunable pore size and structure,as well as good biocompatibility.In this study,we investigated the factors including concentration,solution feeding rate,applied voltage and drying methods that affecting the morphology of PDLLA fibrous scaffolds.The optimum process parameters were determined： solution concentration was 0.20g/mL,solution feeding rate was 0.8mL/h,applied voltage was 12kV.After lyophilization,uniformly distributed PDLLA fibers with diameters of 500-900nm were obtained.In vitro cell morphology observation showed that PDLLA fibrous scaffolds having good cytocompatibility.
参考文献
| [1] | Zong X Z;Kim K;Fang D F et al.Structure and process relationship of eleetrospun bioabsorable nanofaiber membranes[J].Polymer,2002,43:4403-4412. |
| [2] | Xu XY;Yang QB;Wang YZ;Yu HJ;Chen XS;Jing XB .Biodegradable electrospun poly(L-lactide) fibers containing antibacterial silver nanoparticles[J].European Polymer Journal,2006(9):2081-2087. |
| [3] | Fang Mei;Jinsheng Zhong;Xiaoping Yang .Improved Biological Characteristics of Poly(L-Lactic Acid) Electrospun Membrane by Incorporation of Multiwalled Carbon Nanotubes/Hydroxyapatite Nanoparticles[J].Biomacromolecules,2007(12):3729-3735. |
| [4] | Wang, C;Chien, HS;Yan, KW;Hung, CL;Hung, KL;Tsai, SJ;Jhang, HJ .Correlation between processing parameters and microstructure of electrospun poly(D,L-lactic acid) nanofibers[J].Polymer: The International Journal for the Science and Technology of Polymers,2009(25):6100-6110. |
| [5] | Reisea M;Wyrw R;MOiler U et al.Release of metronidazole from electrospun poly (L-lactide-co-D/ITlactide) fibers for local periodontitis treatment[J].Dental Materials,2012,28(02):179-188. |
| [6] | 陈际达,崔磊,刘伟,曹谊林.溶剂浇铸/颗粒沥滤技术制备组织工程支架材料[J].中国生物工程杂志,2003(04):32-35,42. |
| [7] | 赵敏丽,隋刚,杨小平,邓旭亮,胡晓阳.电纺丝PLLA/HA复合纤维支架的制备及体外降解性能研究[J].中国生物医学工程学报,2006(04):476-480. |
| [8] | Flemming RG;Murphy CJ;Abrams GA;Goodman SL;Nealey PF .Effects of synthetic micro- and nano-structured surfaces on cell behavior.[J].Biomaterials,1999(6):573-588. |
| [9] | 葛鹏飞,葛明桥,魏取福,高卫东.聚乳酸纤维的静电纺丝及其形态结构研究[J].合成纤维,2007(01):1-4. |
| [10] | 何晨光,高永娟,赵莉,崔磊,曹谊林.静电纺丝的主要参数对PLGA纤维支架形貌和纤维直径的影响[J].中国生物工程杂志,2007(08):46-52. |
| [11] | Mo XM.;Xu CY.;Kotaki M.;Ramakrishna S. .Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation[J].Biomaterials,2004(10):1883-1890. |
| [12] | H. Fong;I. Chun;D.H. Reneker .Beaded nanofibers formed during electrospinning[J].Polymer: The International Journal for the Science and Technology of Polymers,1999(16):4585-4592. |
| [13] | Yunrong Dai;Junfeng Niu;Lifeng Yin;Jiangjie Xu;Yaohong Xi .Sorption of polycyclic aromatic hydrocarbons on electrospun nanofibrous membranes: Sorption kinetics and mechanism[J].Journal of hazardous materials,2011(3):1409-1417. |
| [14] | Prabhakaran M P;Venugopal J;Ramakrishna S .Electrospun nanostructured scaffolds for bone tissue engineering[J].Acta Biomaterialia,2009,5(08):2884-2893. |
| [15] | 胡雯,黄争鸣,唐亮.PLLA纳米纤维编织缝合线的制备及生物相容性研究[J].功能材料,2009(11):1888-1891,1899. |
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