生物材料组成成分对细胞生物功能有不同的影响。利用静电纺丝技术制备了基于聚己内酯（PCL,polycaprolactone）的不同天然蛋白、多糖（丝素蛋白（SF,silk fibroin）、透明质酸（HA,hyaluronicacid））的混合组分纳米纤维,采用了扫描电镜和接触角对纳米纤维进行基础表征。同时,进一步考察了纳米纤维作为组织工程支架的可行性。研究结果表明SF组分能增加材料的可纺性,有利于细胞的前期黏附,并能够促进细胞增殖。HA组分可以改善材料的亲水性,增加细胞伪足并促进细胞迁移。重要的是,PCL/SF/HA纳米纤维能同时结合SF和HA的优点,有望在组织工程领域得到应用。
The scaffold compositions played different roles on cellular biological functions.In this study,we developed polycaprolactone（PCL） based nanofibers composed of natural protein and polysaccharides（hyaluronan（HA）,silk fibroin（SF）） via electrospinning technology.These nanofibers were characterized by scanning electron microscopy（SEM） and contact angle.Furthermore,we evaluated the potential of different composite nanofibers as tissue engineering scaffolds.In vitro cultivation of human primary skin fibroblasts on the SF-based scaffolds showed a significant increase in cell proliferation.A significant number of cells were found to have well-developed cytoskeleton on PCL/SF nanofibers.Addition of HA component transformed current PCL and PCL/SF components into hydrophilic fibers.Importantly,HA-based scaffolds significantly enhanced cell filopodia protrusions and migration in vitro.PCL/SF/HA nanofibers could combine the advantages of SF and HA.These findings suggest that such multiple blended nanofibers may offer possibilities to tissue engineering application.
参考文献
| [1] | Jonathan Gunn;Miqin Zhang .Polyblend nanofibers for biomedical applications: perspectives and challenges[J].Trends in biotechnology,2010(4):189-197. |
| [2] | Sittinger M;Hutmacher DW;Risbud MV .Current strategies for cell delivery in cartilage and bone regeneration.[J].Current Opinion in Biotechnology,2004(5):411-418. |
| [3] | Schagemann JC;Kurz H;Casper M .The effect of scaffold composition on the early structural characteristics of chondrocytes and expression of adhesion molecules.[J].Biomaterials,2010(10):2798-2805. |
| [4] | Electrospinning of Manmade and Biopolymer Nanofibers-Progress in Techniques, Materials, and Applications[J].Advanced functional materials,2009(18):2863. |
| [5] | Kurella A;Dahotre N B .Review paper:surface modification for bioimplants:the role of laser surface engineering[J].Journal of Biomaterials Applications,2005,20(01):5-50. |
| [6] | Liao Yuzheng;Yang Ping;Wang Jing et al.Behaviour of HUVECs adhered on patterned HA titanium[J].Journal of Functional Materials,2010,41(10):1807-1809. |
| [7] | Anilkumar TV;Muhamed J;Jose A;Jyothi A;Mohanan PV;Krishnan LK .Advantages of hyaluronic acid as a component of fibrin sheet for care of acute wound.[J].Biologicals: Journal of the International Association of Biological Standardization,2011(2):81-88. |
| [8] | Toole B P .Hyaluronan:from extracellular glue to pericellular cue[J].Nature Reviews Cancer,2004,4(07):528-539. |
| [9] | Mandal BB;Kundu SC .Cell proliferation and migration in silk fibroin 3D scaffolds.[J].Biomaterials,2009(15):2956-2965. |
| [10] | Hang Yichun;Zhang Yaopeng;Jin Yuan et al.Preparation and morphology characterization of coaxial electrospun regenerated silk fibroin/sericin fiber[J].Journal of Functional Materials,2011,42(11):2021-2025. |
| [11] | Garcia Fuentes M;Meinel AJ;Hilbe M .Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering.[J].Biomaterials,2009(28):5068-5076. |
| [12] | Schiffman JD;Schauer CL .A review: Electrospinning of biopolymer nanofibers and their applications[J].Polymer reviews,2008(2):317-352. |
| [13] | Altman GH;Diaz F;Jakuba C;Calabro T;Horan RL;Chen J;Lu H;Richmond J;Kaplan DL .Silk-based biomaterials.[J].Biomaterials,2003(3):401-416. |
| [14] | Sofia S;McCarthy M B;Gronowicz G et al.Functionalized silk-based biomaterials for bone formation[J].Journal of Biomedical Materials Research,2001,54(01):139-148. |
| [15] | Cipitria A;Skelton A;Dargaville T R et al.Design,fabrication and characterization of PCL electrospun scarfolds-a review[J].Journal of Materials Chemistry,2011,21(26):9419-9453. |
| [16] | Venugopal J;Low S;Choon AT;Ramakrishna S .Interaction of cells and nanofiber scaffolds in tissue engineering.[J].Journal of biomedical materials research, Part B. Applied biomaterials,2008(1):34-48. |
| [17] | Shao HJ;Lee YT;Chen CS;Wang JH;Young TH .Modulation of gene expression and collagen production of anterior cruciate ligament cells through cell shape changes on polycaprolactone/chitosan blends.[J].Biomaterials,2010(17):4695-4705. |
| [18] | Schindler M;Nur E Kamal A;Ahmed I;Kamal J;Liu HY;Amor N;Ponery AS;Crockett DP;Grafe TH;Chung HY;Weik T;Jones E;Meiners S .Living in three dimensions: 3D nanostructured environments for cell culture and regenerative medicine.[J].Cell biochemistry and biophysics,2006(2):215-227. |
| [19] | Wehrle-Haller B;Imhof B A .Actin,microtubules and focal adhesion dynamics during cell migration[J].International Journal of Biochemistry & Cell Biology,2003,35(01):39-50. |
| [20] | Mattila PK;Lappalainen P .Filopodia: molecular architecture and cellular functions.[J].Nature reviews: molecular cell biology,2008(6):446-454. |
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