以对甲基苯甲酸为唯一碳源筛选能够生物催化合成对苯二甲酸的菌株,发现只有睾丸酮丛毛单胞菌DSM6577可以氧化对甲基苯甲酸生成对苯二甲酸. 对该菌株的细胞生长及其催化对甲基苯甲酸转化为对苯二甲酸的过程进行了研究. 结果表明,底物在8 h内即可完全转化,产物的生成时间为14～31 h, 其中在21 h时产量最大,为34 mg/L.
p-Toluic acid was used as a sole source of carbon and energy to screen for a bacterium to produce terephthalic acid. Comamonas testosterone strain DSM6577 can oxidize p-toluic acid to terephthalic acid through sequential reactions catalyzed by a series of enzymes. The growth of the strain, degradation of p-toluic acid, and production of terephthalic acid were studied. p-Toluic acid can be completely transformed within 8 h, but the formation of terephthalic acid takes place during 14 to 31 h, and a maximal yield of 34 mg/L is obtained at 21 h.
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