In recent life science research, identifying and characterizing entire biomolecular interaction networks is prerequisite to understanding cellular processes on a molecular and biophysical level. A surface plasmon resonance (SPR)imaging interferometer based on spatial phase modulation detection is presented to meet this demand. In this method, the SPR sensing chip surface is illuminated with collimated parallel light beam, and a Wollaston prism is introduced into the reflected light path to produce the interference of polarized light reflected from SPR sensing chip. Information of biomolecular interactions can be obtained by extracting the phase change from the SPR interference patterns. Using our interferometer, we made experiments on a series concentration of NaCl solutions to investigate its detection range, linearity, sensitivity and resolution. The results indicate that the SPR imaging interferometer is mainly sufficient for biomolecular interaction detection.
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