采用碱热处理和仿生沉积HA对注射成形多孔钛进行表面处理,研究改性后多孔钛的细胞毒性及其成骨细胞黏附、增殖和分化的影响.结果表明:改性后多孔钛无细胞毒性,不同孔隙度多孔钛的细胞黏附形貌不同,复合培养7 d后,5%孔隙度组细胞平铺于材料表面,30%孔隙度组细胞多呈长梭形,60%孔隙度组MG63细胞伪足以锚状牢固附着于孔壁的颗粒之上,形成细胞桥;成骨细胞在高孔隙度组表面细胞的增殖数高于低孔隙度组表面细胞的;复合培养7 d与14 d,5%孔隙度组与30%孔隙度组的上清液中碱性磷酸酶(Alkaline phosphatase,ALP)含量无明显差别(p<0.05),而60%孔隙度组的ALP含量高于低孔隙度组的.
参考文献
[1] | MacDonald DE;Rapuano BE;Deo N;Stranick M;Somasundaran P;Boskey AL .Thermal and chemical modification of titanium-aluminum-vanadium implant materials: effects on surface properties, glycoprotein adsorption, and MG63 cell attachment.[J].Biomaterials,2004(16):3135-3146. |
[2] | Ryan G;Pandit A;Apatsidis DP .Fabrication methods of porous metals for use in orthopaedic applications.[J].Biomaterials,2006(13):2651-2670. |
[3] | C. E. Holy;J. A. Fialkov;J. E. Davies .Use of a biomimetic strategy to engineer bone[J].Journal of biomedical materials research, Part B. Applied biomaterials,2003(4):447-453. |
[4] | ITALA A I;YLANEN H O;EKHOLM C;KARLSSON K H ARO H T .Pore diameter of more than 100 micron is not requisite for bone ingrowth in rabbits[J].Journal of Biomedical Materials Research,2001,58(06):679-683. |
[5] | Maitz MF;Poon RWY;Liu XY;Pham MT;Chu PK .Bloactivity of titanium following sodium plasma immersion ion implantation and deposition[J].Biomaterials,2005(27):5465-5473. |
[6] | KOKUBO T;KUSHITANI H;SAKKA S;KITSUGI T YAMAMURO T .Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramics A-W3[J].Journal of Biomedical Materials Research,1990,24(06):721-734. |
[7] | St-Pierre JP;Gauthier M;Lefebvre LP;Tabrizian M .Three-dimensional growth of differentiating MC3T3-E1 pre-osteoblasts on porous titanium scaffolds[J].Biomaterials,2005(35):7319-7328. |
[8] | Sergo V.;Clarke DR.;Sbaizero O. .MECHANICAL AND CHEMICAL CONSEQUENCES OF THE RESIDUAL STRESSES IN PLASMA SPRAYED HYDROXYAPATITE COATINGS[J].Biomaterials,1997(6):477-482. |
[9] | Otsuki B;Takemoto M;Fujibayashi S;Neo M;Kokubo T;Nakamura T .Pore throat size and connectivity determine bone and tissue ingrowth into porous implants: Three-dimensional micro-CT based structural analyses of porous bioactive titanium implants[J].Biomaterials,2006(35):5892-5900. |
[10] | ALTANKOV G;GROTH T .Reorganization of substratum-bound fibronectin on hydrophilic and hydrophobic materials is related to biocompatibility[J].Journal of Mater Med,1994,5(9/10):732-737. |
[11] | Feng B;Chen JY;Qi SK;He L;Zhao JZ;Zhang XD .Carbonate apatite coating on titanium induced rapidly by precalcification.[J].Biomaterials,2002(1):173-179. |
[12] | Zinger O;Anselme K;Denzer A;Habersetzer P;Wieland M;Jeanfils J;Hardouin P;Landolt D .Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography.[J].Biomaterials,2004(14):2695-2711. |
[13] | KIM H M;MIYAJI F;KOKUBO T;NAKAMURA T .Preparation of bioactive Ti and its alloys via simple chemical surface treatment[J].Journal of Biomedical Materials Research,1996,32(03):409-417. |
[14] | Lim JY;Taylor AF;Li Z;Vogler EA;Donahue HJ .Integrin expression and osteopontin regulation in human fetal osteoblastic cells mediated by substratum surface characteristics.[J].Tissue engineering,2005(1/2):19-29. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分:
-
10%
-
20%
-
30%
-
40%
-
50%