建立了高效液相色谱测定维C银翘片中维生素C、对乙酰氨基酚、马来酸氯苯那敏和绿原酸4种组分含量的方法.研究优化了样品提取的溶剂与超声提取条件、色谱分离条件和检测波长等,采用了Sinochrom ODS-BP(4.6 mm×200 mm,5 μm)色谱柱,以0.05 mol/L KH_2PO_4(pH 3.0,含1%三乙胺)-乙腈(75∶25,v/v)为流动相,双检测波长260 nm和326 nm等分离检测条件.结果表明,维生素C、对乙酰氨基酚、马来酸氯苯那敏和绿原酸的测定线性范围均比较宽,方法平均回收率大于99.4% ,相对标准偏差(RSD)小于1.8% (n=5),而且测定方法快速、简便、准确,非常适合维C银翘片类药物的质量控制.
A reversed-phase high performance liquid chromatographic (RP-HPLC) method with dual wavelength detection was developed for the determination of vitamin C,paracetamol,chlorphenamine maleate and chlorogenic acid in Vitamin C Yinqiao Tablets.The separation was performed on a Sinochrom ODS-BP column with the mobile phase consisting of 0.05 mol/L KH _2PO_4 (pH 3.0,containing 1% triethylamine and acetonitrile (75∶25,v/v).The detection wavelength was set at 260 nm (λ_1) and 326 nm (λ_2).The method linearities were wide and the average recoveries were more than 99.4% .The relative standard deviations were less than 1.8% (n=5).This method is convenient,rapid and accurate.It is readily applied to the quality controls in the production process of Vitamin C Yinqiao Tablets.
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