为探讨壳聚糖基共混膜作为组织工程化角膜内皮细胞载体的可行性,制备了羟乙基壳聚糖/明胶/硫酸软骨素共混膜,并评价其性质。结果表明该膜片具有良好的透明度,适宜的含水量;细胞毒性0～1级;体外培养的角膜内皮细胞能够很好地贴附和生长于共混膜上;植入大鼠肌肉内,能够稳定地降解,诱发的炎症反应明显低于不可降解手术缝合线,说明该膜片具有良好的组织相容性,有望作为角膜细胞载体构建组织工程化角膜。
A novel chitosan-based membrane made of hydroxyethyl chitosan,gelatin and chondroitin sulfate was used as a carrier of corneal endothelial cells.The characteristics of the blend membrane,such as transparency,equilibrium water content,cytotoxicity were determined.To study the effects of cell attachment and growth on the membrane,rabbit corneal endothelial cells were cultured on the artificial membrane.And the in vivo biodegradability and histocompatibility of the blend membrane were evaluated by implantation into the muscle of the rats and the anterior chamber of rabbits.As a result,the optical transparency of the membrane was as good as the natural human cornea.The water content of this scaffold was 74.25% which was remarkably close to the native cornea.The membrane is belonged to non-cytotoxicity materials.The cultured rabbit corneal endothelial cells formed a monolayer on the membrane which demonstrated that it was suitable for corneal endothelial cells to attach and grow.In addition,the membrane could be degraded in vivo steadily with less inflammation and showed a good histocompatibility property.These results demonstrated that the hydroxyethyl chitosan-chondroitin sulfate-gelatin blend membrane can potentially be used as a carrier for corneal endothelial cell transplantation.
参考文献
| [1] | Yang J;Yamato M;Shimizu T;Sekine H;Ohashi K;Kanzaki M;Ohki T;Nishida K;Okano T .Reconstruction of functional tissues with cell sheet engineering.[J].Biomaterials,2007(34):5033-5043. |
| [2] | 袁进,周文天.角膜内皮细胞移植的研究进展[J].实用临床医学,2001(01):83-85. |
| [3] | 傅瑶,范先群,曹谊林.组织工程化角膜组织的研究新进展[J].国外医学（眼科学分册）,2001(02):77-81. |
| [4] | 姚子昂,吴海歌,韩宝芹,刘万顺.壳聚糖-透明质酸共混膜性质的研究[J].高技术通讯,2004(09):95-99. |
| [5] | Sung HJ;Meredith C;Johnson C;Galis ZS .The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis.[J].Biomaterials,2004(26):5735-5742. |
| [6] | Laschke MW;Haufel JM;Thorlacius H;Menger MD .New experimental approach to study host tissue response to surgical mesh materials in vivo.[J].Journal of biomedical materials research, Part A,2005(4):696-704. |
| [7] | 陈列欢,刘万顺,韩宝芹,位晓娟,杨朝忠.角膜内皮细胞载体膜片的性质研究及移植试验[J].高等学校化学学报,2006(05):880-884. |
| [8] | 傅瑶,陈苹,范先群.异种角膜脱细胞基质和几丁糖载体构建角膜基质层的比较[J].眼科研究,2006(06):561-564. |
| [9] | 王迎军,赵晓飞,卢玲,任力,陈晓峰,葛坚,刘炳乾.角膜组织工程支架壳聚糖-胶原复合膜的性能[J].华南理工大学学报（自然科学版）,2006(08):1-5. |
| [10] | Rafat M;Li F;Fagerholm P;Lagali NS;Watsky MA;Munger R;Matsuura T;Griffith M .PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering.[J].Biomaterials,2008(29):3960-3972. |
| [11] | 位晓娟,刘万顺,韩宝芹,陈列欢,杨朝忠.兔角膜内皮细胞载体的体外培养及移植的研究[J].中国生物医学工程学报,2006(05):590-595. |
| [12] | 高兴爽,刘万顺,韩宝芹,位晓娟.壳聚糖基角膜细胞载体的制备及其细胞相容性[J].生物工程学报,2008(08):1381-1386. |
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