以pannonibacter phragmitetusT1菌作为生物吸附剂,研究细菌代谢活性、培养时间、溶液pH值、菌体用量、温度、吸附时间、初始金属浓度等因素对其吸附重金属pb2+的影响,并进一步研究吸附机理.结果表明,培养12h的灭活T1菌体在pb2+浓度为150 mg/L、pH值为6、T1菌添加量为0.5 g/L、温度为30℃的条件下,对Pb2+的最大吸附量为68.35 mg/g;灭活T1菌对重金属Pb2+的吸附过程较快,90 min即达到吸附平衡,pb2+的吸附符合Pseudo-second order模型和Langmuir等温吸附模型.结合红外光谱分析,灭活pannonibacter phragmitetus T1 菌对Pb2+是表面吸附,羟基和酰胺基是与Pb2+络合反应的主要基团.
参考文献
| [1] | WANG J L;CHEN C .Biosorption of heavy metals by Saccharomyces cerevisia:A review[J].Biotechnology Advances,2006,24:427-451. |
| [2] | Vijayaraghavan K;Yun YS .Bacterial biosorbents and biosorption.[J].Biotechnology Advances: An International Review Journal,2008(3):266-291. |
| [3] | 潘蓉,曹理想,张仁铎.青霉菌和镰刀菌对重金属Cd~（2+）、Cu~（2+）、Zn~（2+）和Pb~（2+）的吸附特性[J].环境科学学报,2010(03):477-484. |
| [4] | RIORDAN C;MCHALE A P .Removal of lead from solution using non-living residual brewery yeast[J].BioprocessEngineering,1998,19:277-280. |
| [5] | FAN T;LIU Y;FENG B Y;ZENG G M YANG C P ZHOU M ZHOU H Z TAN Z F WANG X .Biosorption of cadmium(Ⅱ),zinc(Ⅱ) and lead(Ⅱ) by Penicillium simplicissimum:Isotherms,kinetics and thermodynamics[J].Journal of Hazardous Materials B:Environmental Technologies,2008,160:655-661. |
| [6] | HANG Z L;ZENG G M;HUANG D L;LI L F HUANG P M XIA C B .Adsorption of lead(Ⅱ) from aqueous solution onto Hydrilla verticillata[J].Biodegradation,2009,20:651-660. |
| [7] | 代群威,董发勤,M.J.NOONAN,张伟.面包酵母菌在铅铜模拟废水中的吸附动力学[J].中国有色金属学报,2010(04):788-794. |
| [8] | Bohumil Volesky .Biosorption and me[J].Water research: A journal of the international water association,2007(18):4017-4029. |
| [9] | Ahluwalia SS;Goyal D .Microbial and plant derived biomass for removal of heavy metals from wastewater[J].Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies,2007(12):2243-2257. |
| [10] | Sisca O. Lesmana;Novie Febriana;Felycia E. Soetaredjo .Studies on potential applications of biomass for the separation of heavy metals from water and wastewater[J].Biochemical Engineering Journal,2009(1):19-41. |
| [11] | 许旭萍,沈雪贤,陈宏靖.球衣菌吸附重金属Hg2+的理化条件及其机理研究[J].环境科学学报,2006(03):453-458. |
| [12] | 关晓辉,秦玉春,王立文,尹荣,鲁敏,杨迎军.纳米Fe3O4负载的浮游球衣菌去除重金属离子的工艺研究[J].环境科学,2007(02):436-440. |
| [13] | VOLESKY B;PHILLIPS A M .Biosorption of heavy metals by Saccharomyces cerevisiae[J].Applied Microbiology and Biotechnology,1995,42(05):797-806. |
| [14] | 陈志英,王磊,周琪.菌龄对恶臭假单胞菌吸附铜离子能力的影响[J].中国环境科学,2006(z1):97-101. |
| [15] | Bala Kiran;Anubha Kaushik;C.P. Kaushik .Biosorption of Cr(VI) by native isolate of Lyngbya putealis (HH-15) in the presence of salts[J].Journal of hazardous materials,2007(3):662-667. |
| [16] | So-Young Kang;Jong-Un Lee;Kyoung-Woong Kim .Biosorption of Cr(III)and Cr(VI)onto the cell surface of Pseudomonas aeruginosa[J].Biochemical Engineering Journal,2007(1):54-58. |
| [17] | 朱一民,周东琴,魏德州.啤酒酵母菌对汞离子（Ⅱ）的生物吸附[J].东北大学学报（自然科学版）,2004(01):89-91. |
| [18] | Zulkali MMD;Ahmad AL;Norulakmal NH .Oryza sativa L. husk as heavy metal adsorbent: Optimization with lead as model solution[J].Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies,2006(1):21-25. |
| [19] | Chen XC;Wang YP;Lin Q;Shi JY;Wu WX;Chen YX .Biosorption of copper(II) and zinc(II) from aqueous solution by Pseudomonas putida CZ1[J].Colloids and Surfaces, B. Biointerfaces,2005(2):101-107. |
| [20] | 陈灿,王建龙.酿酒酵母吸附Zn2+、Pb2+、Ag+、Cu2+的动力学特性研究[J].环境科学学报,2007(04):544-553. |
| [21] | HO Y S;MCKAY G .Batch lead(Ⅱ) removal from aqueous solution by peat:Equilibrium and kinetics[J].Transactions of the Institution of Chemical Engineers,1999,77(B):165-173. |
| [22] | OZTURK A;ARTAN T;AYAR A .Biosorption of nickel (Ⅱ) and copper (Ⅱ) ions from aqueous solution by Streptomyces coelicolor A3(2)[J].Colloids and Surfaces B:Biointerfaces,2004,34(02):105-111. |
| [23] | Loukidon, MX;Karapantsios, TD;Zouboulis, AI;Matis, KA .Cadmium(II) biosorption by Aeromonas caviae: Kinetic modeling[J].Separation Science and Technology,2005(6):1293-1311. |
| [24] | 卢涌泉;邓振华.实用红外光谱解析[M].北京:电子工业出版社,1989:21-163. |
| [25] | 代群威,董发勤.FTIR技术在几株细菌鉴定中的应用[J].西南科技大学学报,2009(01):114-118. |
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