蛋白质分子的三维结构是生命科学研究中极为重要的信息,X射线单晶衍射技术是目前获得结构信息最主要的手段,但如何筛选到第一个蛋白晶体是该技术必需的第一步,也是制约结构生物学发展的主要瓶颈问题之一.现在一般通过规模筛选的方法从众多的溶液中筛选出可结晶的条件,但是工作量较大,效率也不高.回顾了近年来在提高结晶筛选效率方向取得的成就,主要表现在2个方面:一是在传统结晶方法和试剂基础上发展起来的一系列新的高效的结晶技术和筛选试剂盒;二是从生物学、物理学和化学等角度提出的一些提高结晶筛选效率的新技术,主要包括通过分子工程改造蛋白、提高蛋白溶液的稳定和均一性、导入籽晶、共结晶、改善结晶界面和变温筛选等技术.最后展望了该领域未来的发展趋势.
参考文献
| [1] | Segelke B W .Efficiency analysis of sampling protocols used in protein crystallization screening[J].Journal of Crystal Growth,2001,232(1-4):553. |
| [2] | Chayen NE;Saridakis E .Protein crystallization: from purified protein to diffraction-quality crystal[J].Nature methods,2008(2):147-153. |
| [3] | Baldock P.;Stewart PS.;Mills V. .A COMPARISON OF MICROBATCH AND VAPOUR DIFFUSION FOR INITIAL SCREENING OF CRYSTALLIZATION CONDITIONS[J].Journal of Crystal Growth,1996(1/4):170-174. |
| [4] | D'Arcy A;Mac Sweeney A;Stihle M;Haber A .The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions.[J].Acta crystallographica.Section D. Biological crystallography,2003(Pt.2):396-399. |
| [5] | Talreja S;Kim DY;Mirarefi AY;Zukoski CF;Kenis PJA .Screening and optimization of protein crystallization conditions through gradual evaporation using a novel crystallization platform[J].Journal of Applied Crystallography,2005(6):988-995. |
| [6] | Nneji GA;Chayen NE .A crystallization plate for controlling evaporation in hanging drops[J].Journal of Applied Crystallography,2004(3):502-503. |
| [7] | Ng JD.;Gavira JA.;Garcia-Ruiz JM. .Protein crystallization by capillary counterdiffusion for applied crystallographic structure determination[J].Journal of Structural Biology,2003(1):218-231. |
| [8] | Gareia-Ruiz J M;Gonzalcz-Ramirez L A;Gavira J A et al.Granada erystallisation box:A new device for protein crystallisation by counter-diffusion techniques[J].Acta Crystallographica Section D:Biological Crystallography,2002,58:1638. |
| [9] | Gonzalez-Ramirez LA;Carrera J;Gavira JA;Melero-Garcia E;Garcia-Ruiz JM .Granada Crystallization Facility-2: A Versatile Platform for Crystallization in Space[J].Crystal growth & design,2008(12):4324-4329. |
| [10] | Sommer MOA;Larsen S .Crystallizing proteins on the basis of their precipitation diagram determined using a microfluidic formulator[J].Journal of synchrotron radiation,2005(Pt.6):779-785. |
| [11] | Carl L. Hansen;Emmanuel Skordalakes;James M. Berger;Stephen R. Quake .A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion[J].Proceedings of the National Academy of Sciences of the United States of America,2002(26):16531-16536. |
| [12] | Caffrey M. .Membrane protein crystallization[J].Journal of Structural Biology,2003(1):108-132. |
| [13] | Ehud M. Landau;Jurg P. Rosenbusch .Lipidic cubic phases: A novel concept for the crystallization of membrane proteins[J].Proceedings of the National Academy of Sciences of the United States of America,1996(25):14532-14535. |
| [14] | Faham S;Bowie JU .Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure.[J].Journal of Molecular Biology,2002(1):1-6. |
| [15] | C. E. Kundrot .Which strategy for a protein crystallization project?[J].Cellular and molecular life sciences: CMLS,2004(5):525-536. |
| [16] | Sulzenbacher G;Gruez A;Roig Zamboni V;Spinelli S;Valencia C;Pagot F;Vincentelli R;Bignon C;Salomoni A;Grisel S;Maurin D;Huyghe C;Johansson K;Grassick A;Roussel A;Bourne Y;Perrier S;Miallau L;Cantau P;Blanc E;Genevois M;Grossi A;Zenatti A;Campanacci V;Cambillau C .A medium-throughput crystallization approach.[J].Acta crystallographica.Section D. Biological crystallography,2002(Pt.12):2109-2115. |
| [17] | Majeed S.;Ofek G.;Belachew A.;Huang CC.;Zhou TQ.;Kwong PD. .Enhancing protein crystallization through precipitant synergy[J].Structure,2003(9):1061-1070. |
| [18] | Alexander McPherson;Chieniang Nguyen;Steven B. Larson;John S. Day;Bob Cudney .Development of an alternative approach to protein crystallization[J].Journal of structural and functional genomics,2007(4):193-198. |
| [19] | McPherson A;Cudney B .Searching for silver bullets: An alternative strategy for crystallizing macromolecules[J].Journal of Structural Biology,2006(3):387-406. |
| [20] | Gao W;Li SX;Bi RC .An attempt to increase the efficiency of protein crystal screening: a simplified screen and experiments.[J].Acta crystallographica.Section D. Biological crystallography,2005(Pt.6):776-779. |
| [21] | Hui R.;Edwards A. .High-throughput protein crystallization[J].Journal of Structural Biology,2003(1):154-161. |
| [22] | Kantardjieff KA;Rupp B .Protein isoelectric point as a predictor for increased crystallization screening efficiency[J].Bioinformatics,2004(14):2162-2168. |
| [23] | 袁彩,刘钐,黄明东.分子生物学在蛋白质结晶中的应用[J].生物化学与生物物理进展,2005(02):111-115. |
| [24] | Bianchi E;Venturini S;Pessi A et al.High level expression and rational mutagenesis of a designed protein,the minibody:From an insoluble to a soluble molecule[J].Journal of Molecular Biology,1994,236(02):649. |
| [25] | Derewenda ZS .The use of recombinant methods and molecular engineering in protein crystallization.[J].Methods: A Companion to Methods in Enzymology,2004(3):354-363. |
| [26] | Cusack S .Large-scale induced fit recognition of an m(7)GpppG cap analogue by the human nuclear cap-binding complex.[J].EMBO Journal,2002(20):5548-5557. |
| [27] | Derewenda U;Mateja A;Devedjiev Y;Routzahn KM;Evdokimov AG;Derewenda ZS;Waugh DS .The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague[J].Structure,2004(2):301-306. |
| [28] | Walter TS;Meier C;Assenberg R;Au KF;Ren JS;Verma A;Nettleship JE;Owens RJ;Stuart DI;Grimes JM .Lysine methylation as a routine rescue strategy for protein crystallization[J].Structure,2006(11):1617-1622. |
| [29] | Kim Y;Quartey P;Li H et al.Large-scale evaluation of protein reductive methylation for improving protein crystallization[J].Nature Methods,2008,5(10):853. |
| [30] | YANNIS GEORGALIS;JENS SCHUELER;JOACHIM FRANK;MARIO DIKEOS SOUMPASIS;WOLFRAM SAENGER .PROTEIN CRYSTALLIZATION SCREENING THROUGH SCATTERING TECHNIQUES[J].Advances in Colloid and Interface Science,1995(1):57-86. |
| [31] | Borgstahl G .How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals[J].Methods in Molecular Biology,2007,363:109. |
| [32] | Pusey M L.Screening for crystallization conditions-Just the FACTs[A].Mexico,2008:52. |
| [33] | Bergfors T. .Seeds to crystals[J].Journal of Structural Biology,2003(1):66-76. |
| [34] | Ireton GC;Stoddard BL .Microseed matrix screening to improve crystals of yeast cytosine deaminase[J].Acta crystallographica.Section D. Biological crystallography,2004(Pt.3):601-605. |
| [35] | D'Arcy A;Frederic VA;Marsh M .An automated microseed matrix-screening method for protein crystallization[J].Acta crystallographica.Section D. Biological crystallography,2007(Pt.4):550-554. |
| [36] | Schartman RR .On the thermodynamics of cocrystal formation.[J].International Journal of Pharmaceutics,2009(1/2):77-80. |
| [37] | Mosbacher TG;Bechthold A;Schulz GE .Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes.[J].Journal of Molecular Biology,2005(3):535-545. |
| [38] | Kevin P. Madauss;Su-Jun Deng;Robert J. H. Austin;Millard H. Lambert;Iain McLay;John Pritchard;Steven A. Short;Eugene L. Stewart;Ian J. Uings;Shawn P. Williams .Progesterone Receptor Ligand Binding Pocket Flexibility: Crystal Structures of the Norethindrone and Mometasone Furoate Complexes[J].Journal of Medicinal Chemistry,2004(13):3381-3387. |
| [39] | Mazumdar M;Zhou M;Zhu DW;Azzi A;Lin SX .Crystallogenesis of steroid-converting enzymes and their complexes: Enzyme-ligand interaction studies and inhibitor design facilitated by complex structures[J].Crystal growth & design,2007(11):2206-2212. |
| [40] | Sakamoto Y;Kaneda M;Terasaki O et al.Direct imaging of the pores and cages of three-dimensional mesoporous materials[J].Nature,2000,408(6811):449. |
| [41] | Chayen NE;Saridakis E;El-Bahar R;Nemirovsky Y .Porous silicon: an effective nucleation-inducing material for protein crystallization[J].Journal of Molecular Biology,2001(4):591-595. |
| [42] | Naomi E. Chayen;Emmanuel Saridakis;Richard P. Sear .Experiment and theory for heterogeneous nucleation of protein crystals in a porous medium[J].Proceedings of the National Academy of Sciences of the United States of America,2006(3):597-601. |
| [43] | Zhu DW;Garneau A;Mazumdar M;Zhou M;Xu GJ;Lin SX .Attempts to rationalize protein crystallization using relative crystallizability[J].Journal of Structural Biology,2006(3):297-302. |
| [44] | Zhang CY;Yin DC;Lu QQ;Guo YZ;Guo WH;Wang XK;Li HS;Lu HM;Ye YJ .Cycling Temperature Strategy: A Method to Improve the Efficiency of Crystallization Condition Screening of Proteins[J].Crystal growth & design,2008(12):4227-4232. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%