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选取高低两个LET点(40和60 keV/μm),剂量点分别为50,100,200,400,600 Gy进行辐照处理,研究了生防菌的存活率与突变率的关系,抑菌谱以及活性等.结果表明,在高LET条件下,低剂量辐照就可以得到较多的突变体,并且BJ1有较高的存活率和突变谱,有利于筛选优良的正突变体.因此高LET较低LET有更为明显的辐射诱变效应.

To explore the effects of different linear energy transfer(LET)of ~(12)C~(6+) ion Beam irradiation on BJ1, survivals and biocontrol effects were cultured and treated with LET= 40 and 60 keV/μm at the doses of 50, 100, 200, 400 and 600 Gy, respectively. The results showed more mutations and biocontrol charts and higher survivals were obtained with high LET(60 keV/μm) irradiations at lower dose, which was useful to screen good positive mutations. Based on the results above, it could be concluded that the condition of high LET(60 keV/μm) had obvious mutagenic effects than that of low LET(40 keV/μm).

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