Effect of Rare Earths on Composition and Activities of Rare Earth Elements Binding Glycoprotein in Tea

稀土学报(英文版), 2001, 19(2): 125-128.

汪东风 ^1, , 李俊 ^2, , 赵贵文 ^3, , 王常红 ^4, , 魏正贵 ^5, , 尹明 ^6,

1.Department of Chemistry, University of Science and Technology of China;Key Laboratory of Tea Biotechnology, Anhui Agricultural University, Agricultural Ministry of the People′s Republic of China

2.Institute of Clinical Pharmacology, Anhui Medical University,

3.Department of Chemistry, University of Science and Technology of China,

4.Key Laboratory of Tea Biotechnology, Anhui Agricultural University, Agricultural Ministry of the People′s Republic of China,

5.Department of Chemistry, University of Science and Technology of China,

6.Institute of Rock and Mineral Analysis, Chinese Academy of Geological Science,

基金项目: 国家自然科学基金(29805003) 国家自然科学基金(97412004)

Effect of Rare Earths on Composition and Activities of Rare Earth Elements Binding Glycoprotein in Tea

The effects of spraying rare earths(RE) on composition and activities of tea polysaccharide were measured by inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography(GC), amino acid analyzer and animal models. The results show that there are rare earth elements binding glycoprotein in tea (REE-TGP). The effects of RE on composition and content of saccharides in REE-TGP are not obvious. The contents of Hypro and Ser in REE-TGP are evidently enhanced in comparison with that in control (not treated with rare earth), but the content of Glu is smaller than that from control. The content of La in REE-TGP from the tea garden sprayed rare earth is 193% higher than that in control. REE-TGP declines content of blood sugar in mice and enhances immunization of rat, which are very evident when the animals are treated by REE-TGP from the tea garden sprayed RE.

参考文献

[1]	WANG Dong-feng;XIE Xiao-feng;WANG Ying-long .Studying progress in tea polysacchride and its pharmacology[J].Natural Product Research and Development(in Chinese),1996,8(01):63-68.
[2]	ZHONG Shuo-ling;MING Wei-zong.A rare earths binding lipopolysacchride isolated from leaves in tea plant[J].科学通报,2002(09):863.
[3]	ZHANG Wei-jie.Biochemical Techniques of Complex Carbohydrate (in Chin )[M].上海:上海科学技术出版社,1987:209-225.
[4]	Mizumo T;HAGIWARA T;NAKMURA T et al.Antitumor activity and some proporties of water-soluble polysacchrides from Himematsutake”, the fruiting body of agaricus blazei murill[J].Agricultural and Biological Chemistry,1990,54(11):2889-1896.
[5]	TIAN Geng·yuan;WANG Cheng;FENG Yu cheng .Isolation, purification and properties of LbGP and characterization of its glycan-peptide bond[J].生物化学与生物物理学报,2002,27(02):201-205.
[6]	CAO Xin-de;ZHAO Gui-wei;YIN Ming.Determination of ultrace rare earth elements in tea by inductively coupled plasma mass spectrometry with microwave digestion and AG50w-x8 action exchange chromatography[J].ANALYST,1998(123):1115-1119.
[7]	WANG Dong-feng;TANG Hua-rong;ZHAO Gui-wen .Hydroxyproline-rich glycoprotein (HRGP) separation and identification in tea leaves and its inducing by rare earths[J].Journal of Tea Sci(in Chin),1998,18(02):119-124.
[8]	PERPER R J;ALVAREZ B;COLOMBO C.Theuseofstandardizedadjuvantarthriticsassaytodifferentiatebetweenanti-inflammatoryandimmunosuppressiveagent[J].Proceedings of the Society for Experimental Biology and Medicine,1971(137):506-511.
[9]	Yamamoto I;OOHMORI H;SESANO M .ConA-induced proliferative response of splenocytes from arthritic rat[J].Drugs under Experimental and Clinical Research,1982,8(01):5-8.
[10]	DING Gui-feng;XI Hong-li;DENG Yu-lang et al.Measurement of IL-2 by activating mouse spleen cells[J].Journal Shanghai Immunology(in Chin),1988,8(01):64-67.
[11]	LIANG Jun-shan;WEI Wei;ZHOU Ai wu et al.Method for measuring IL-1 and effects of total glucosides of peony on IL-1 production[J].中国药理学通报,2002,5(06):354-357.

上一张下一张

计量

下载量()
访问量()

作者相关

文章评分

您的评分：
1

0%
2

0%
3

0%
4

0%
5

0%

材料期刊网

Effect of Rare Earths on Composition and Activities of Rare Earth Elements Binding Glycoprotein in Tea

参考文献