The Influence of Nano-apatite on c-myc and p53 Gene in the Hepatocellular Carcinoma

武汉理工大学学报-材料科学版(英文版), 2005, 20(2): 57-59.

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1.The First Hospital,Wuhan University of Technology,Wuhan 430070,China

2.Biomedical Material and Engineering Center, Wuhan University of Technology,Wuhan 430070,China

3.Biomedical Material and Engineering Center, Wuhan University of Technology,Wuhan 430070,China

4.Biomedical Material and Engineering Center, Wuhan University of Technology,Wuhan 430070,China

5.Biomedical Material and Engineering Center, Wuhan University of Technology,Wuhan 430070,China

基金项目: Funded by the State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology(WUT2004Z02) 湖北省科技创新项目(2002AA105A)

The Influence of Nano-apatite on c-myc and p53 Gene in the Hepatocellular Carcinoma

Keywords： apatite

The influence mechanism of the nano-apatite on the human hepatocellular carcinoma in vitro was investigated. Using the homogeneous precipitation method, the nano-apatite was synthesized at room temperature, and it was characterized with transmission electron microscopy (TEM) and the Zataplus. The influence on the expression of the c-myc and p53 gene in the human hepatocellular carcinoma cell lines were tested with the TEM and hybridization in situ. The TEM and the Zataplus analyses show that the nano-apatite is distributed homogenously in size and needle-shaped sizes, which ranges from 67.5 nm to 88.3 nm. It is found that the nano-apatitet increases the volume of the human hepatocellular carcinoma cells, makes extensive cytoplasmic vacuolization, the mitochondria swelling, chromatin in nucleus dispersed partially and condensed around the nuclear membranes.The interspace in nuclear membranes were separated and even the cytoplasm dissolved. It is also found that the expression of the c-myc gene is inhibited, but the p53 is enhanced. The experimental results demonstrate that the nano-apatite enables the oncosis of the human hepatocellular carcinoma cells by down-regulation of the expression of the c-myc and up-regulation of the expression of the p53 in vitro.

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The Influence of Nano-apatite on c-myc and p53 Gene in the Hepatocellular Carcinoma

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