将自体富集骨髓间充质干细胞(MSCs),快速复合多孔β磷酸三钙(β-TCP),构建生物活性复合材料(MSCs/β-TCP).通过分层随机分组对照比较了MSCs/β-TCP与单纯β-TCP及自体骨在羊腰椎后路融合的效果.测定富集后碱性磷酸酶染色为阳性的细胞集落单位(CFUs/ALP+,即MSCs数量)变化情况.用扫描电镜观察了MSCs与β-TCP短期复合情况.通过X线、CT扫描和组织学观察对比植入材料成骨情况及转归.结果表明,富集后MSCs数量增加约3.9倍,短期复合2h后MSCs即可在多孔β-TCP内壁黏附;X线检查,MSCs/β-TCP组与自体骨组的融合区逐渐有中高密度影形成,CT扫描测定MSCs/β-TCP组与自体骨组的融合率,分别为92%和100%,明显高于单纯β-TCP组(58%).硬组织形态计量学证实,复合材料MSCs/β-TCP与自体骨的骨再生能力相当,优于单纯β-TCP材料.
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