用含铕稀土配合物单体成功地合成出含铕稀土荧光聚合物(PPNEu).通过荧光光谱仪、紫外可见分光光度计研究了含铕稀土荧光聚合物与牛血清蛋白(BSA)的相互作用机理.结果表明:含铕稀土荧光聚合物能够与BSA相互结合,并使BSA出现荧光猝灭现象,该猝灭效应属于静态猝灭;结合常数和结合位点数分别为:KA=8.49×104 L/mol,n=1.25(298 K);KA =7.45×104 L/mol,n=1.25(308 K);在与BSA的结合过程中,吉布斯自由能(△G)为负值,熵变(△S)为正值且焓变(△H)为负值,说明静电力在PPNEu与BSA结合过程中起着主要作用.基于F(o)rster理论,结合距离为3.38 nm.
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