在多肽EAK16水凝胶支架上接种小鼠前成骨细胞MC3T3-E1,采用倒置显微镜观察细胞形态,CCK-8(细胞计数试剂盒)检测细胞增殖情况.细胞在诱导培养基中培养1周后,观察不同时间段细胞碱性磷酸酶的分泌活性.采用ALP染色和茜素红-S染色作为定性实验研究MC3T3-E1向成骨方向的分化情况.结果表明,MC3T3-E1细胞在水凝胶支架EAK16上有较好的黏附和增殖能力,诱导培养后细胞有较高水平的碱性磷酸酶表达和矿化基质沉积.多肽水凝胶支架对前成骨细胞MC3T3-E1具有较好的生物相容性.
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