随着微加工技术的发展,在材料表面构筑功能化的纳微米级图案越来越成为关注的热点.在生物科学领域,光刻蚀、微接触印刷和蘸笔纳米平板印刷等微图案技术被广泛应用并实现了生物分子在几十纳米微区域上的固定.生物分子在微纳米区域内的成功固定大大推动了生物微分析、生物芯片、生物微器件等生物技术及相关领域的发展.本文从分析生物分子与材料表面的相互作用入手,较为系统地评述了构筑生物分子微图案的几种重要方法,并对生物分子微图案技术的发展做了展望.
As the development of micro fabrication technologies,the construction of functional micropatterns on the surfaces of materials has become one of the most important subjects of current research.In bioscience field,selective localization of biomolecules to micropatterns or defined positions is very important for the development of bioassay,biochip,biomicrodevices etc.The biopatterns with the feature size of tens nanometers,which have been prepared with photolithography,microcontact printing and dip-pen nanolithography etc,greatly promotes the development of many biotechnologies.This paper systematically reviews the above-mentioned methods by analyzing the interactions between bio-molecules and the surfaces of materials and describes the outlook of this technology at last.
参考文献
| [1] | Zhu,H;Bilgin,M;Bangham,R;Hall,D;Casamayor,A;Bertone,P;Lan,N;Jansen,R;Bidlingmaier,S;Houfek,T;Mitchell,T;Miller,P;Dean,RA;Gerstein,M;Snyder,M .Global analysis of protein activities using proteome chips.[J].Science,2001(5537):2101-2105. |
| [2] | Zhou DJ.;Wang XZ.;Birch L.;Rayment T.;Abell C. .AFM study on protein immobilization on charged surfaces at the nanoscale: Toward the fabrication of three-dimensional protein nanostructures[J].Langmuir: The ACS Journal of Surfaces and Colloids,2003(25):10557-10562. |
| [3] | Nyamjav D;Ivanisevic A .Templates for DNA-templated Fe(3)O(4) nanoparticles.[J].Biomaterials,2005(15):2749-2757. |
| [4] | Ramunas Valiokas;Sarunas Vaitekonis;Goran Klenkar;Gediminas Trinkonas;Bo Liedberg .Selective Recruitment of Membrane Protein Complexes onto Gold Substrates Patterned by Dip-Pen Nanolithography[J].Langmuir: The ACS Journal of Surfaces and Colloids,2006(8):3456-3460. |
| [5] | Sigal GB.;Whitesides GM.;Mrksich M. .Effect of surface wettability on the adsorption of proteins and detergents[J].Journal of the American Chemical Society,1998(14):3464-3473. |
| [6] | Jinyho Hyun;Woo-Kyung Lee;Nidhi Nath;Ashutosh Chilkoti;Stefan Zauscher .Capture and Release of Proteins on the Nanoscale by Stimuli-Responsive Elastin-Like Polypeptide"Switches"[J].Journal of the American Chemical Society,2004(23):7330-7335. |
| [7] | Lee SW;Oh BK;Sanedrin RG;Salaita K;Fujigaya T;Mirkin CA .Biologically active protein nanoarrays generated using parallel dip-pen nanolithography[J].Advanced Materials,2006(9):1133-1136. |
| [8] | Robinson WilliamH;DiGennaro Carla;Hueber Wolfgang;Haab BrianB;Kamachi Makoto;Dean ErikJ;Fournel Sylvie;Fong Derek;Genovese MarkC;de-Vegvar-HenryE Neuman;Skriner Karl;Hirschberg DavidL;Morris RobertI;Muller Sylviane;Pruijn GerJ;van-Venrooij WaltherJ;Smolen JosefS;Brown PatrickO;Steinman Lawrence;Utz PaulJ .Autoantigen microarrays for multiplex characterization of autoantibody responses.[J].Nature medicine,2002(3):295-301. |
| [9] | Cha T;Guo A;Zhu XY .Enzymatic activity on a chip: the critical role of protein orientation[J].Proteomics,2005(2):416-419. |
| [10] | 晏海英,姚树寅,吴仲岿,陈红.聚甲基丙烯酸甲酯薄膜表面的亚微米级图案化[J].材料导报,2009(08):11-13. |
| [11] | Zhongkui Wu;Haiying Yan;Hong Chen;He Huang .One-stage Fabrication Of Sub-micron Hydrophilic Microchannels On Pdms[J].Applied Surface Science: A Journal Devoted to the Properties of Interfaces in Relation to the Synthesis and Behaviour of Materials,2009(8):4702-4704. |
| [12] | Hayashi K.;Saito N.;Sugimura H.;Takai O.;Nakagiri N. .Regulation of the surface potential of silicon substrates in micrometer scale with organosilane self-assembled monolayers[J].Langmuir: The ACS Journal of Surfaces and Colloids,2002(20):7469-7472. |
| [13] | Hong L.;Sugimura H.;Furukawa T.;Takai O. .Photoreactivity of alkylsilane self-assembled monolayers on silicon surfaces and its application to preparing micropatterned ternary monolayers[J].Langmuir: The ACS Journal of Surfaces and Colloids,2003(6):1966-1969. |
| [14] | Yoshiko Miura;Hajime Sato;Takayasu Ikeda;Hiroyuki Sugimura;Osamu Takai;Kazukiyo Kobayashi .Micropatterned Carbohydrate Displays by Self-Assembly of Glycoconjugate Polymers on Hydrophobic Templates on Silicon[J].Biomacromolecules,2004(5):1708-1713. |
| [15] | Yamazoe H;Uemura T;Tanabe T .Facile cell patterning on an albumin-coated surface[J].Langmuir: The ACS Journal of Surfaces and Colloids,2008(16):8402-8404. |
| [16] | Sugiura S;Edahiro JI;Sumaru K;Kanamori T .Surface modification of polydimethylsiloxane with photo-grafted poly(ethylene glycol) for micropatterned protein adsorption and cell adhesion[J].Colloids and Surfaces, B. Biointerfaces,2008(2):301-305. |
| [17] | Odom TW.;Love JC.;Wolfe DB.;Paul KE.;Whitesides GM. .Improved pattern transfer in soft lithography using composite stamps[J].Langmuir: The ACS Journal of Surfaces and Colloids,2002(13):5314-5320. |
| [18] | Li HW.;Muir BVO.;Fichet G.;Huck WTS. .Nanocontact printing: A route to sub-50-nm-scale chemical and biological patterning[J].Langmuir: The ACS Journal of Surfaces and Colloids,2003(6):1963-1965. |
| [19] | Inerowicz HD.;Howell S.;Regnier FE.;Reifenberger R. .Multiprotein immunoassay arrays fabricated by microcontact printing[J].Langmuir: The ACS Journal of Surfaces and Colloids,2002(13):5263-5268. |
| [20] | Csucs G.;Kunzler T.;Feldman K.;Robin F.;Spencer ND. .Microcontact printing of macromolecules with submicrometer resolution by means of polyolefin stamps[J].Langmuir: The ACS Journal of Surfaces and Colloids,2003(15):6104-6109. |
| [21] | Jinho Hyun;Sang Jung Ahn;Woo Kyung Lee;Ashutosh Chilkoti;Stefan Zauscher .Molecular Recognition-Mediated Fabrication of Protein Nanostructures by Dip-Pen Lithography[J].Nano letters,2002(11):1203-1207. |
| [22] | Lee KB;Park SJ;Mirkin CA;Smith JC;Mrksich M .Protein nanoarrays generated by dip-pen nanolithography[J].Science,2002(5560):1702-1705. |
| [23] | Lee M;Kang DK;Yang HK;Park KH;Choe SY;Kang C;Chang SI;Han MH;Kang IC .Protein nanoarray on Prolinker (TM) surface constructed by atomic force microscopy dip-pen nanolithography for analysis of protein interaction[J].Proteomics,2006(4):1094-1103. |
| [24] | Demers LM;Ginger DS;Park SJ;Li Z;Chung SW;Mirkin CA .Direct patterning of modified oligonucleotides on metals and insulators by dip-pen nanolithography[J].Science,2002(5574):1836-1838. |
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