目的:借助于MINITAB统计学软件,采用Plaekett-Burman设计和响应面优化法对重组类人胶原蛋白Ⅱ(RHLCⅡ)仿生人工骨材料成型工艺进行优化研究.方法:首先利用Plackett-Burman设计筛选出对材料抗压强度和孔隙率影响显著的三个因素,即纳米羟基磷灰石(nHA)/RHLCⅡ、京尼平浓度和预冷温度.在此基础上用最陡爬坡试验逼近最大响应区域,找到后续试验的中心点.再利用Box-Behnken实验设计及响应面分析法进行回归分析,确定主要影响因素的最佳水平.结果:优化后的三种主要影响成分为nHA/RHLCⅡ=1.54、京尼平浓度0.9%和预冷温度-75℃.结论:方程拟合良好,优化后的材料机械强度和孔隙率分别为81.56MPa、94.5%,较优化前分别提高了1.11MPa和0.9%;MTT测定结果表明材料细胞毒性试验合格,符合骨组织工程支架材料的要求,重复性好,有望应用于商品化生产.
Plackett-Burman design and Response Surface Methodology (RSM) were used to optimize a molding technology of human-like collagenⅡ(RHLCⅡ)biomimetic composite.Methods:It showed by Plackett-Burman Experimental Design that nHA/RHLC Ⅱ,the concentration of genipin and precooling temperature had significant effects on the strength and pore size of seafrold.Then the path of steepest ascent was carried out to approach the optimal region of those three factors.Box-Behnken design and RSM were employed to investigate the interactions of the variables,and to ascertain the optimaI values of the factors,which finally led to the maximum mechanical properties of the material.Results:The optimal concentrations of the factors were:HA/RHLCⅡ 1.54,Genipin 0.9% and precooling temperature-75℃.Conclusion:The results showed a good fitness with the model.Mechanical strength and porosity were 81.56MPa and 94.5%,which were 1.1 Mpa and 0.9% higher than previous work respectively.The MTT results showed a good biocompatibility and high reproductivity of the RHLCⅡ scaffold.Therefore,it is thought to be an ideal material for bone tissue regeneration and commercilization.
参考文献
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