利用固液相分离与冷冻干燥技术分别制备明胶与PLGA组织工程支架。两种支架微观呈互相连通的多孔结构,孔隙率相似。 PLGA支架的压缩模量达到3.22MPa,约为明胶支架的500倍。明胶支架在8周内完全降解,而PLGA支架在8周的降解时间内,质量损失在5%以下。
Gelatin scaffold and PLGA scaffold were fabricated via solid liquid phase separation technique and freeze drying method.These scaffolds displayed interconnected porous microstructures and the porosities were nearly the same.The compressive modulus of PLGA scaffold was 3.22MPa which was about 500 times more than that of gelatin scaffold.In vitro degradation test demonstrated that gelatin scaffold degraded completely while PLGA scaf-fold only lost less than 5%of its original mass during 8 weeks.
参考文献
[1] | Liu Y;Lim J;Teoh S H .Development of clinically relevant scaffolds for vascularised bone tissue en-gineering[J].Biotechnology Advances,31(05):688-705. |
[2] | Autissier A;Visage CL;Pouzet C;Chaubet F;Letourneur D .Fabrication of porous polysaccharide-based scaffolds using a combined freeze-drying/cross-linking process.[J].Acta biomaterialia,2010(9):3640-3648. |
[3] | Arafat MT;Lam CX;Ekaputra AK;Wong SY;Li X;Gibson I .Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering.[J].Acta biomaterialia,2011(2):809-820. |
[4] | Wray L S;Rnjak-Kovacina J;Mandal B B et al.A silk-based scaffold platform with tunable archi-tecture for engineering critically-sized tissue[J].BIOMATERIALS,33(36):3914-3926. |
[5] | Okamoto M;John B .Syntheitc biopolymer nano-composites for tissue engineering scaffold[J].Pro-gress in Polymer Science,38(10):1487-1503. |
[6] | Duarte ARC;Mano JF;Reis RL .Preparation of chitosan scaffolds loaded with dexamethasone for tissue engineering applications using supercritical fluid technology[J].European Polymer Journal,2009(1):141-148. |
[7] | Ang T H;Sultana S A;Hutmacher D W et al.Fabrication of 3 D chitosan-hydroxyatite scaffolds using a robotic dispensing system[J].Materials Science and Engineering C,20(01):35-42. |
[8] | Yuan, W.;Feng, Y.;Wang, H.;Yang, D.;An, B.;Zhang, W.;Khan, M.;Guo, J. .Hemocompatible surface of electrospun nanofibrous scaffolds by ATRP modification[J].Materials science & engineering, C. Materials for Biogical applications,2013(7):3644-3651. |
[9] | 刘磊,吴若峰.聚乳酸类材料的水解特性[J].合成材料老化与应用,2006(01):44-48. |
[10] | Zhu, J.;Xiong, Y.;Zeng, C.;Qiang, N.;Quan, D.;Wan, J. .Elastic chitosan conduits with multiple channels and well defined microstructure[J].International Journal of Biological Macromolecules: Structure, Function and Interactions,2012(1/2):105-112. |
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