建立了阿折地平对映体的高效液相色谱拆分方法.采用Chiralpak AD-H (250 mm×4.6 mm,5.0 μm,Daicel公司)手性色谱柱在正相条件下直接拆分阿折地平对映体,考察了固定相种类、流动相组成及柱温等对阿折地平对映体分离的影响.确定了最佳的拆分条件:流动相为正己烷-异丙醇(90∶10,v/v),流速为0.8 mL/min,检测波长为254 nm;柱温为20 ℃;在此条件下阿折地平对映体的分离度为3.3.该法简单快速,重现性好.
A high performance liquid chromatographic (HPLC) method was established for the enantiomeric separation of azelnidipine.Baseline chiral separation of azelnidipine was achieved under normal-phase chromatographic mode using the Chiralpak AD-H chiral column (250 mm×4.6 mm,5.0 μm,Daicel).The influences of the nature of chiral stationary phase,form of the mobile phase,and column temperature on the enantiomeric separation were studied.The optimized chromatographic conditions were hexane-isopropyl alcohol (90∶10,v/v) as the mobile phase with a flow rate of 0.8 mL/min and detection at the wavelength of 254 nm.The column temperature was set at 20 ℃.The resolution of 3.3 for azelnidipine was achieved under the above chromatographic conditions.The method is simple,rapid and with good reproducibility.
参考文献
| [1] | Zhang K,Xue N,Du Y M,et al.Chinese Journal of Pharmaceuticals (张恺,薛娜,杜玉民,等.中国医药工业杂志),2008,39(3):163 |
| [2] | He Y H.World Clinic Drugs,2004,25(10):636 |
| [3] | Chen A H,Wu R,Tao X.Chinese Journal of New Drugs and Clinical Remedies (陈爱华,邬蓉,陶霞.中国新药与临床杂志),2006,25(12):946 |
| [4] | Wellington K,Scott L J.Drugs,2003,63(23):2613 |
| [5] | Huang Z Y.Chinese Journal of New Drugs and Clinical Remedies (黄仲义.中国新药与临床杂志),2008,27(3):236 |
| [6] | Kiyoshi K,Naozumi S,Yoko U,et al.J Chromatogr B,2007,852:389 |
| [7] | Ding L.Chromatographic Analysis for Drugs.Beijing:People's Medical Publishing House (丁黎.药物色谱分析.北京:人民卫生出版社),2008 |
| [8] | Tang Q,Chen X Y.Chinese Journal of Analytical Chemistry (唐琴,陈先勇.分析化学),2007,35(2):240 |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%