通过化学交联法合成组氨酸修饰透明质酸耦合物(His-HA),制备载阿霉素纳米粒,分析其pH值响应性和抗肿瘤特征.研究显示,随着pH值的降低(7.4～5.5),纳米粒的粒径增大(230～780nm),zeta电位升高,载药纳米粒的体外释放量增加.细胞毒性实验显示粒径＜300nm的载药纳米粒具更高的毒性.细胞摄入实验表明,阿霉素通过受体介导的胞吞和载药纳米粒的胞外释放两种途径被细胞摄入.以上研究显示组氨酸修饰透明质酸纳米粒具有显著的pH值响应性,具备作为阿霉素药物载体的应用前景.
参考文献
| [1] | Platt,VM;Szoka-FC,Jr .Anticancer Therapeutics: Targeting Macromolecules and Nanocarriers to Hyaluronan or CD44, a Hyaluronan Receptor.[J].Molecular Pharmaceutics,2008(4):474-486. |
| [2] | Yadav AK;Mishra P;Agrawal GP .An insight on hyaluronic acid in drug targeting and drug delivery.[J].Journal of drug targeting,2008(2):91-107. |
| [3] | Saravanakumar G;Choi KY;Yoon HY;Kim K;Park JH;Kwon IC;Park K .Hydrotropic hyaluronic acid conjugates: Synthesis, characterization, and implications as a carrier of paclitaxel.[J].International Journal of Pharmaceutics,2010(1/2):154-161. |
| [4] | Cho HJ;Yoon IS;Yoon HY;Koo H;Jin YJ;Ko SH;Shim JS;Kim K;Kwon IC;Kim DD .Polyethylene glycol-conjugated hyaluronic acid-ceramide self-assembled nanoparticles for targeted delivery of doxorubicin.[J].Biomaterials,2012(4):1190-1200. |
| [5] | Shen Y;Tang H;Radosz M .pH-responsive nanoparticles for cancer drug delivery[J].Methods in Molecular Biology,2008,437:183-216. |
| [6] | Lai JJ;Hoffman JM;Ebara M;Hoffman AS;Estournes C;Wattiaux A;Stayton PS .Dual magnetic-/temperature-responsive nanoparticles for microfluidic separations and assays[J].Langmuir: The ACS Journal of Surfaces and Colloids,2007(13):7385-7391. |
| [7] | Lee ES;Gao ZG;Kim D;Park K;Kwon IC;Bae YH .Super pH-sensitive multifunctional polymeric micelle for tumor pH(e) specific TAT exposure and multidrug resistance[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2008(3):228-236. |
| [8] | Chen-Guang Liu;Xi-Guang Chen;Hyun-Jin Park .Self-assembled nanoparticles based on linoleic-acid modified chitosan:Stability and adsorption of trypsin[J].Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides,2005(3):293-298. |
| [9] | Park K;Lee GY;Kim YS;Yu M;Park RW;Kim IS;Kim SY;Byun Y .Heparin-deoxycholic acid chemical conjugate as an anticancer drug carrier and its antitumor activity[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2006(3):300-306. |
| [10] | Wong HL;Rauth AM;Bendayan R;Manias JL;Ramaswamy M;Liu Z;Erhan SZ;Wu XY .A new polymer-lipid hybrid nanoparticle system increases cytotoxicity of doxorubicin against multidrug-resistant human breast cancer cells.[J].Pharmaceutical research,2006(7):1574-1585. |
| [11] | Zhao S R;Liu C G .Preparation of self assembled sodiu(rn) alginate nanoparticles and the study on a nano delivery vehicle for rotenone[J].Journal of Functional Materials,2010,9:1543-1546. |
| [12] | Oishi M;Nagasaki Y .Stimuli-responsive smart nanogels for cancer diagnostics and therapy.[J].Nanomedicine,2010(3):451-468. |
| [13] | Muro S;Wiewrodt R;Thomas A;Koniaris L;Albelda SM;Muzykantov VR;Koval M .A novel endocytic pathway induced by clustering endothelial ICAM-1 or PECAM-1.[J].Journal of Cell Science,2003(Pt.8):1599-1609. |
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