由于低能离子较低的组织穿透能力,其诱变机理一直是研究者争论的问题。近年来,本研究组的一系列研究工作已经证明在植物中存在辐射远程（诱变）效应,从一个新的角度解释了低能离子的诱变机理,然而依然无法解释低能离子辐照中的许多独特生物现象,而这些现象均具有明显的表观遗传学特性。以表观遗传学最具标志性特征的胞嘧啶甲基化为研究对象,以α粒子-拟南芥菜根辐照实验体系作为研究平台,检测了远程组织（器官）甲基化相关基因AtDML3的表达及特定基因片段的甲基化水平。研究证实,在植物个体水平辐射可以诱导远程表观遗传的变化,为进一步探索低能离子的诱变机理提供了新的思路。
Along the way,the mutagenic mechanism of low-energy ions irradiation is a debatable issue.Recently,the existence of radiation induced long-range（mutagenic） effects in vivo in plants has been performed in a series of studies of our group,which account for the mutagenesis of low-energy ions irradiation in a new perspective.However,numerous distinct biology phenomena remain to be addressed,which bear obvious characteristics to epigenetic.In the present study,using the expression of methylation-related AtDML3 gene and methylation level of specific gene segments as end points,the methylation of cytosine,the most important feature of epigenetic,was investigated.It was shown that,in A.thaliana,root-loca-lized α-irradiation could induce epigenetic changes in aerial parts which avoided the direct irradiation.The radiation induced long-range epigenetic changes were confirmed in this study,which supplied innovative ideas for the further investigation of the mutagenetic mechanism of low-energy ions irradiation.
参考文献
| [1] | 余增亮.离子束生物技术引论.合肥:安徽科技出版社,1998. |
| [2] | Yu Zengliang. An Introduction of Ion Beam Biotechnology. New York: Springer Press, 2006. |
| [3] | 卞坡,张艳峰,吴健,等.郑州大学学报,2001,33:45. |
| [4] | 韩建伟,余增亮.生物物理学报,1998,14:757. |
| [5] | 卞坡,霍裕平,秦广雍,等.生物物理学报,1999,15:551. |
| [6] | Wei Zengquan, Xie Hongmei, Han Guangwu, et al. Nucl Instr and Meth, 1995, B95: 371. |
| [7] | Wei Zengquan, Xie Hongmei, Han Guangwu, et al. Nucl Instr and Meth, 1998, B114: 191. |
| [8] | Yang Gen, Wu Lijun, Chen Lianyun, et al. Radiation Research, 2007, 167: 298. |
| [9] | Yang Gen, Mei Tao, Yuan Han, et al. Radiation Research, 2008, 170: 372. |
| [10] | 刘萍,李方华,徐敏,等.原子核物理评论,2008,25(2):191. |
| [11] | Li Fanghua, Liu Ping, Wang Ting, et al. Radiation Research, 2010, 174: 228. |
| [12] | 王卫东.离子注入小麦诱变剂量效应及转化后代遗传特性研究[D].郑州:郑州大学,2004(9). |
| [13] | Ya Huiyuan, Gu Yunhong, Jiao Zhen, et al. Journal of Plant Physiology and Molecular Biology, 2007, 33: 507. |
| [14] | Li Fanghua, Liu Ping, Wang Ting, et al. Radiation Research, 2010, 174:228. |
| [15] | Sedelnikova O A, Nakamura A. Cancer Research, 2007, 67: 4295. |
| [16] | Koturbash I, Rugo R E, Hendricks C A. Oncogene, 2006, 25: 4267. |
| [17] | Koturbash I, Boyko A, Rodriquez-Juarez R. Carcinogenesis, 2007, 28: 1831. |
| [18] | Li Fanghua, Wang Ting, Xu Shuyan, et al. International Journal of Radiation Biology(in press). |
| [19] | Molinier J, Ries G, Zipfel C, et al. Nature, 2006, 442: 1046. |
| [20] | Kovalchuk I, Baulch J E. Environmental and Molecular Mutagenesis, 2008, 49: 16. |
| [21] | Boyko A, Kovalchuk I. Environmental and Molecular Mutagenesis, 2008, 49: 61. |
| [22] | Ortega-Galisteo A P, Morales-Ruiz T, Ariza R R, et al. Plant Molecular Biology, 2008, 67: 671. |
| [23] | 王婷,李方华,徐淑艳,等.原子核物理评论,2010,27(4):488. |
| [24] | Weigel D, Glazebrook J. Arabidopsis: a Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 2002, 249-252. |
| [25] | Yu Hong, Chen Xi, Hong Yuanyuan, et al. Plant Cell, 2008, 20: 1134. |
| [26] | Grunau C, Clark S J, Rosenthal A. Nucleic Acids Research, 2001, 29(13): e65. |
| [27] | Gruntman E, Qi Y J, Slotkin R K, et al. BMC Bioinformat ics, 2008, 9: 371. |
| [28] | Miura A, Nakamura M, Inagaki S, etal. The EMBO Journal, 2009, 28: 1078. |
| [29] | He X J, Chen T P, Zhu J K. Cell Research, 2011, 21: 442. |
| [30] | Cokus S J, Feng S H, Zhang X Y, et al. Nature, 2008, 452 (13) : 215. |
| [31] | Gruenbaum Y, Naveh Many T, Cedar H, et al. Nature, 1981, 292: 860. |
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