综述了3D细胞培养技术（TDCC）的发展,3种主要的体外组织构建方法;辐射诱导2D细胞产生旁效应的现象与机理;人工构建的3D组织辐射后诱导的旁效应及其细胞间信号传导机理。重离子（C离子）辐照作为一种重要的放疗工具,对其辐射处理3D组织后诱导产生的旁效应进行了展望。由于3D组织更接近人体细胞生长的真实环境,因而以3D组织作为模型研究辐射诱导的旁效应,对于辐射旁效应的防护和治疗可能具有重要的指导意义。
Compared with the cultured monolayer（2D） cells,three-dimensional（3D） tissue could be more similar to the environment in vivo including the physical support,chemical factors,cell-cell and cell-matrix interaction and so on.With the development of three-dimensional cell culture techniques（TDCC）,3D tissue is widely used in the areas of bystander effect research.This review focuses on introducing the TDCC method and its application in bystander-effect research.First,the development process of 3D tissue culture method was introduced.Secondly,the induction of radiation induced bystander effects both in 2D cell and 3D tissue and its mechanisms were reviewed.Finally,because heavy ion（carbon ion beam） has been developed as a useful tool to cure solid cancer,and the 3D tissue model is an ideal material to study the damages on body after being irradiated and to understand the underlying mechanisms,future study about heavy ion radiation inducing bystander effect in 3D tissue was discussed.
参考文献
| [1] | EMERMAN J T,PITELKA D R.In Vitro,1977,13(5):316. |
| [2] | AZZAM E I,LITTLE J B.Hum Exp Toxicol,2004,23(2):61. |
| [3] | BEHRAVESH E,EMAMI K,WU H,etal.Adv Space Res,2005,35(2):260. |
| [4] | HEI T K,ZHOU H,IVANOV V N,etal.J Pharm Pharma-col,2008,60(8):943. |
| [5] | WEIGELT B,BISSELL M J.Semin Cancer Biol,2008,18(5):311. |
| [6] | BIRGERSDOTTER A,SANDBERG R,ERNBERG I.SeminCancer Biol,2005,15(5):405. |
| [7] | GRIFFITH L G,SWARTZ M A.Nat Rev Mol Cell Biol,2006,7(3):211. |
| [8] | CUKIERMAN E,PANKOV R,STEVENS D R,etal.Sci-ence,2001,294(5547):1708. |
| [9] | HICKS K O,PRUIJN F B,SECOMB T W,etal.J NatlCancer Inst,2006,98(16):1118. |
| [10] | GELAIN F,BOTTAI D,VESCOVI A,etal.PLoS One,2006,1:e119. |
| [11] | BOKHARI M,CARNACHAN R J,CAMERON N R,etal.Biochem Biophys Res Commun,2007,354(4):1095. |
| [12] | YAMADA K M,CUKIERMAN E.Cell,2007,130(4):601. |
| [13] | GAHWILER B H,CAPOGNA M,DEBANNE D,etal.Trends Neurosci,1997,20(10):471. |
| [14] | SAKAI T,LARSEN M,YAMADA K M.Nature,2003,423(6942):876. |
| [15] | SMALLEY K S,LIONI M,HERLYN M.In Vitro Cell DevBiol Anim,2006,42(8/9):242. |
| [16] | MEIER F,NESBIT M,HSU M Y,etal.Am J Pathol,2000,156(1):193. |
| [17] | BERKING C,HERLYN M.Histol Histopathol,2001,16(2):669. |
| [18] | NAGASAWA H,LITTLE J B.Cancer Res,1992,52(22):6394. |
| [19] | MOTHERSILL C,SEYMOUR C B.Nat Rev Cancer,2004,4(2):158. |
| [20] | MATSUMOTO H,HAYASHI S,HATASHITA M,etal.Radiat Res,2001,155(3):387. |
| [21] | SHAO C,FOLKARD M,MICHAEL B D,etal.Proc NatlAcad Sci USA,2004,101(37):13495. |
| [22] | AZZAM E I,DE TOLEDO S M,LITTLE J B.Proc NatlAcad Sci USA,2001,98(2):473. |
| [23] | IYER R,LEHNERT B E,SVENSSON R.Cancer Res,2000,60(5):1290. |
| [24] | PERSAUD R,ZHOU H,BAKER S E,etal.CRR AnnualReport of Columbia University,2005,17. |
| [25] | BISHAYEE A,RAO D V,HOWELL R W.Radiat Res,1999,152(1):88. |
| [26] | BISHAYEE A,HILL H Z,STEIN D,etal.Radiat Res,2001,155(2):335. |
| [27] | TARTIER L,GILCHRIST S,BURDAK-ROTHKAMM S,etal.Cancer Res,2007,67(12):5872. |
| [28] | SHAO C,STEWART V,FOLKARD M,etal.Cancer Res,2003,63(23):8437. |
| [29] | CHEN S,ZHAO Y,HAN W,etal.Br J Cancer,2008,98(11):1839. |
| [30] | PENAFUERTE C,GALIPEAU J.Cancer Immunol Immu-nother,2008,57(8):1197. |
| [31] | KOVALCHUK O,ZEMP F J,FILKOWSKI J N,etal.Carci-nogenesis,2010,31(10):1882. |
| [32] | BELYAKOV O V,FOLKARD M,MOTHERSILL C,etal.Radiat Prot Dosimetry,2002,99(1-4):249. |
| [33] | BELYAKOV O V,MITCHELL S A,PARIKH D,etal.ProcNatl Acad Sci USA,2005,102(40):14203. |
| [34] | SEDELNIKOVA O A,NAKAMURA A,KOVALCHUK O,etal.Cancer Res,2007,67(9):4295. |
| [35] | PONNAIYA B,MARINO S,GEARD C R,etal.Investiga-tion of Radiation-induced Bystander Responsed in ArtificialSkin Tissues.CRR Annual Report of Columbia University2005,28. |
| [36] | PONNAIYA B,JENKINS-BAKER G,BRENNER D J,etal.Radiat Res,2004,162(4):426. |
| [37] | SCHETTINO G,JOHNSON G W,MARINO S A,etal.IntJ Radiat Biol,2010,86(7):593. |
| [38] | MONTEIRO-RIVIERE N A,INMAN A O,SNIDER T H,etal.Microsc Res Tech,1997,37(3):172. |
| [39] | HYBBINETTE S,BOSTROM M,LINDBERG K.Exp Der-matol,1999,8(1):30. |
| [40] | 郭传玲,王菊芳,金晓东,等.核技术,2006,29(8):587. |
| [41] | SHAO C,AOKI M,FURUSAWA Y.Radiat Environ Bio-phys,2003,42(3):183. |
| [42] | CHU J H,YU S,HAYWARD S W,etal.Prostate,2009,69(4):428. |
| [43] | ASAITHAMBY A,HU BURONG,DELGADO O,etal.Nu-cleic Acids Res,2011,39(13):5474. |
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