以含Ca~(2+)和PO_4~(3-)的溶液为无机相,壳聚糖(chitosan,CS)溶液为高分子相,采用原位水化法制备羟基磷灰石(hydroxyapatite,HAP)/CS复合多孔支架材料.XRD和IR的表征和分析表明水化24h后,复合支架中的钙磷盐从磷酸氢钙(dicalcium phosphate dehydrate,DCPD)转化为HAP.SEM和EDS显示15μm左右的棒状HAP颗粒均匀地分散在多孔支架的孔壁上,压缩强度的测试结果表明这种结构显著提高复合支架的力学性能.
Hydroxyapatite(HAP)/chitosan(CS) composite is a biocompatible and bioactive biocomposite.In order to enhance the properties of the composite,a novel homogenous HAP/CS composite scaffold was developed via lyophilization and in situ hydration.The morphologies,components,mechanical strength,bioactivity and biocompatibility of the composite scaffolds were investigated.The HAP particles with relatively homogenous distribution in the pore-wall surface of the scaffold were obtained while the scaffold remained sponge structure after hydration treatment.X-ray diffraction (XRD) and Fourier-Transformed Infrared spectroscopy (FTIR) confirmed the phase purity of the formed HAP crystals.The compressive strength of the scaffold showed much higher than those of the scaffold blended and the CS-only scaffold.The results suggest that the homogenous composite scaffolds with better strength can be prepared via the in situ hydration process,which may serve as a good scaffold for bone tissue engineering.
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