采用熔融聚合法直接合成生物降解材料聚乳酸-乙醇酸(PLGA),并以其为载体制备基因重组人干扰素聚乳酸-乙醇酸微球(IFN-α-PLGA-MS).通过正交试验对干扰素微球的制备工艺进行了优选,同时对微球的性质进行了考察.结果以D, L-乳酸、乙醇酸为原料,氯化亚锡(SnCl2)为催化剂,在165 ℃、70 Pa下熔融聚合10 h ,PLGA特性粘数[η]最高可达0.2382 dL/g,干扰素微球的形态圆整,平均粒径为10.71 μm,药物确已被包裹在微球中,微球载药量和包封率分别为8.06%、36.97%,且获得了较满意的干扰素微球制备工艺.
参考文献
| [1] | Diwan M;Park TG .Stabilization of recombinant interferon-alpha by pegylation for encapsulation in PLGA microspheres.[J].International Journal of Pharmaceutics,2003(1/2):111-122. |
| [2] | Dong CM.;Gu ZW.;Feng XD.;Qiu KY. .Synthesis of poly(D,L-lactic acid-alt-glycolic acid) from D,L-3-methylglycolide[J].Journal of Polymer Science, Part A. Polymer Chemistry,2000(23):4179-4184. |
| [3] | Wang N;Wu X S;Lujan-Upton H.Polymeric Materials Science and Engineering[A].,1997:373-374. |
| [4] | Zhou S;Deng X;He S;Li X;Jia W;Wei D;Zhang Z;Ma J .Study on biodegradable microspheres containing recombinant interferon-alpha-2a.[J].Journal of Pharmacy and Pharmacology,2002(9):1287-1292. |
| [5] | 唐有根;蒋金芝;王要武.[J].化学世界,1997(11):586-589. |
| [6] | 汪朝阳,赵耀明,王浚,杨帆,韩芸.无毒催化剂催化合成外消旋聚乳酸及其在药物缓释微球中的应用[J].功能高分子学报,2002(04):377-382. |
| [7] | Kim TH;Lee H;Park TG .Pegylated recombinant human epidermal growth factor (rhEGF) for sustained release from biodegradable PLGA microspheres.[J].Biomaterials,2002(11):2311-2317. |
| [8] | Dogar V;Sokhey J;Kumar S;Sood DK;Tahlan AK .Interference of thiomersal in biologicals during protein estimation.[J].Biologicals: Journal of the International Association of Biological Standardization,2002(4):271-275. |
| [9] | 麦杭珍,汪朝阳,严冰,赵耀明.乳酸直接聚合的热力学、动力学研究[J].化学研究与应用,2002(05):534-536. |
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