为评价自制纳米羟基磷灰石/聚氨基酸(n-HA/PAA)复合材料体外细胞相容性,采用MG63成骨样细胞与材料共同培养,以n-HA为对照进行以下实验:①细胞形态学观察;②细胞吸光度值的MTT检测;③细胞碱性磷酸酶(ALP)活力检测;④细胞骨钙素(OCN)含量的酶联免疫法(ELISA)检测;⑤流式细胞仪检测细胞周期及细胞凋亡情况.结果表明,n-HA/PAA复合材料对MG63细胞形态无明显影响,材料周围及表面细胞贴壁生长良好,并随时间的延长细胞逐渐增多.MTT检测细胞吸光度值随培养时间的延长而逐渐增加,且细胞增殖明显,实验组与对照组间各时间点比较无明显差异(P＞0.05).细胞ALP活力及OCN含量随培养时间的延长逐渐增加,实验组与对照组比较无明显差异(P＞0.05).两组材料对细胞的生长周期及凋亡均无明显影响,且两组细胞周期及凋亡细胞各自比较无明显差异(P＞0.05).
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