用荧光法来监视多个生理参数时,需要几个不同的荧光探针分子.这些探针分子要被同一波长激发,但是具有明显分离的、不同的发射波长.目前,大多数荧光探针只有小的斯托克位移(50-90 nm),从而限制了它们在多个物质同时检测上的应用.在这项工作中,我们提出了一个新的分子探针设计:受体-荧光分子1-间隔-荧光分子2(简称RFSF探针).该RFSF探针具有更大的斯托克位移,在对多个物质同时检测时,它可以用来与传统的探针互补.在我们合成的这个分子中,荧光1是萘,荧光2是蒽,这个分子可以用来检测氢质子.在没有氢质子时,萘被280 nm光激发,由于能量转移,在420 nm处观察到了蒽的微弱发射光.此斯托克位移是140 nm,远大于传统的荧光探针分子,比如萘甲基胺的60 nm.当加入氢质子时,蒽在420 nm的荧光增加.此结果表明,一代新的RFSF荧光探针分子可以与传统的探针分子同时用来检测金属离子、代谢物、蛋白质,以及不同的生物标记,它们为荧光检测提供了更广泛的探针分子的选择.
Monitoring multiple physiological parameters by using the fluorescence method requires the loading of several distinct fluorescent probes which are excitable at the same fixed wavelength with well-separated emissions.Most current fluorescent probes only have small Stoke's shift (50-90 nm),which limits their application in detecting multiple species simultaneously.In this work,we report a molecular probe based on a new design of receptor-Fluorophore-1-SpacerFluorophore-2 (RFSF).The RFSF probe has a large Stoke' s shift,which is complementary to the traditional probes in detecting dual species.In this molecule,the fluorophore 1 is naphthalene and the fluorophore 2 is anthracene and the molecule can be used to detect proton.In the absence of proton,when the naphthalene is excited at 280 nm,a weak emission at 420 nm from anthracene due to energy transfer is observed.The Stoke's shift was 140 nm,which was much greater than general fluorescent sensors with a single fluorophore,such as 60 nm for aminemethylnaphthalene.When exposed to protons,the fluorescence of anthracene at 420 nm increases.The results showed suggest that a new family of fluorescent RFSF sensors can be synthesized for biologically important chemicals,including metal ions,metabolites,proteins,and different biomarkers in the future.These valuable assay systems would benefit greatly from a wider choice of fluorescence probes with the same excitation wavelength.
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