建立了用于饲料添加剂阿散酸质量控制的反相高效液相色谱法.采用的色谱条件:Waters Bondapak C_(18)柱(150 mm×4 mm,5 μm)分离,以甲醇-水(用稀磷酸调节pH至2.9) (1∶4,v/v)作流动相,流速1.0 mL/min,紫外检测波长为244 nm.在优化的色谱条件下,阿散酸和掺假物质对氨基苯磺酸在 3 min 内实现了基线分离.阿散酸和对氨基苯磺酸的线性范围均为5～200 mg/L,检出限(S/N=3)分别为0.20 mg/L 和0.15 mg/L.该方法简便快速,适合饲料添加剂阿散酸的分析以及监测对氨基苯磺酸掺假.
A reversed-phase high performance liquid chromatographic (RP-HPLC) method was established for the determination of arsanilic acid and sulfanilic acid as adulterant in the feed additives.The separation was carried out on a Waters Bondapak C_(18) column,and methanol-water (pH 2.9 adjusted by 0.01 mol/L phosphoric acid) (1∶4,v/v) was used as the mobile phase with a flow rate of 1.0 mL/min.A diode array detector was used at 244 nm as the detection wavelength.Arsanilic acid and sulfanilic acid were separated within 3 min.The linear ranges all were 5-200 mg/L and the detection limits (S/N=3) were 0.20 and 0.15 mg/L for arsanilic acid and sulfanilic acid,respectively.This method is simple and rapid,and suitable for the simultaneous determination of arsanilic acid and sulfanilic acid in feed additives.
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