构建了PNIPAAm-g-EVA/明胶支架复合人工皮肤样品,并初步考察了该人工皮肤的细胞相容性。由于明胶支架的生物相容性已经得到充分验证,主要选择成纤维细胞为实验细胞,PNIPAAm-g-EVA膜为研究对象。采用含有胶原酶的消化液消化组织块,在较短时间内可获得足够数量的成纤维细胞。成纤维细胞与PNIPAAm-g-EVA膜共培养结果表明,成纤维细胞在膜表面粘附力强,生长、增殖情况良好。说明与PNIPAAm-g-EVA膜共培养并未影响细胞的正常生理功能,PNIPAAm-g-EVA具有良好的细胞相容性。
In this study a gelatin/PNIPAAm-g-EVA bilayer artificial skin had been constructed and its cell com- patibility had also been assessed. As the good biocompatibility of gel had already been proved. So we focus on assessing the cell compatibility of PNIPAAm-g-EVA membrane. Rat skin fibroblasts were cultured in vitro with PNIPAAm-g-EVA membrane for different periods, and the results indicated that the proliferation of fibro- blasts on PNIPAAm-g-EVA membrane increased with time significantly. Imnunohistochemical staining of fi- broblasts were positive. Therefore, it can be concluded that the PNIPAAm-g-EVA membrane has good cyto- compatibility on the basis of positive effect on the attachment, proliferation and growth of fibroblasts.
参考文献
| [1] | Skeist H.Handbook of adhesives[M].New York:Van Nostrand Reinhold Company Inc,1977:484. |
| [2] | Langer R;Brem H;Tapper D .Biocompatibility of poly- meric delivery systems for maeromolecules[J].Journal of Biomedical Materials Research,1981,15:267-277. |
| [3] | Niemi S M;Fox J G;Brown L R et al.Evaluation of ethyl- ene-vinyl acetate[J].Lab Anita Sci,1985,35:609-612. |
| [4] | Shastri P V .Toxicology of polymers for implant contra- ceptives for women[J].Contraception,2002,65:9-13. |
| [5] | Johnson J C.Sustained release medications[M].Park Ridge:Noyes Data Co,1980:96. |
| [6] | Ocak F;Agabeyoglu I .Development of a membrane-controlled transdermal therapeutic system containing isosorbide dinitrate.[J].International Journal of Pharmaceutics,1999(2):177-183. |
| [7] | Morimoto Y;Seki T;Sugibayashi K et al.Basic studies on controlled transdermal delivery of nicardipine hydro- chloride using ethylene-vinyl acetate and ethylen-vinyi al- cohol copolymer membranes[J].Chemical and Pharmaceutical Bulletin,1988,36:2633-2641. |
| [8] | 周莉玲,侯海霞,李锐.EVA膜对青蒿琥酯经皮渗透影响的研究[J].中药新药与临床药理,2002(02):108-110. |
| [9] | 高珊,赵莉,崔磊,曹谊林.壳聚糖-明胶支架材料的制备及性能研究[J].组织工程与重建外科杂志,2005(06):316-318,357. |
| [10] | Rahul D P;James E M .Preparation solubilization and biodegradation of erosslinked gelatin[J].Polymer Science Part A:Polymer Chemistry,2005,38:786-796. |
| [11] | Lee SB;Kim YH;Chong MS;Hong SH;Lee YM .Study of gelatin-containing artificial skin V: fabrication of gelatin scaffolds using a salt-leaching method.[J].Biomaterials,2005(14):1961-1968. |
| [12] | Ma Yan;Zhang Wenqing;Feng Huafeng et al.Prepa- ration and properties of chitosan-based porous mere branes[J].Journal of Functional Materials,2010,41(03):483-486. |
| [13] | Veelken H;Jesuiter H;Mackensen A et al.Primary fibroblasts from human adults as target cells for ex vivo transfection and gene therapy[J].Human Gene Therapy,1994,5:1203-1210. |
| [14] | Mosmann T .Rapid colorimetric assay for cellular growth and survival:application to proliferation and cy- totoxieity assays[J].Journal of Immunological Methods,1983,65:55-63. |
| [15] | Freimoser FM;Jakob CA;Aebi M;Tuor U .The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay is a fast and reliable method for colorimetric determination of fungal cell densities[J].Applied and Environmental Microbiology,1999(8):3727-3729. |
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