{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":4,"startPagecode":1},"records":[{"abstractinfo":"长链非编码RNA(lncRNA)是长度超过200 nt、不具有蛋白编码功能的转录本，它们在真核生物中发挥基因调控等重要作用。迄今为止，还没有检索到有关参与电离辐射应答的lncRNA研究报道。据推测，细胞暴露于电离辐射之后有些lncRNA的转录水平将发生变化。因此，采用2 Gy的X射线辐照HeLa细胞，辐照后4 h提取总RNA进行全序列测序。最终发现1028种lncRNA的转录水平发生了改变，其中包括381种新lncRNA。从中随机挑选24种lncRNA，用实时荧光定量PCR法验证了2 Gy的X射线或80 keV/μm碳离子束照射后的表达变化，发现其变化趋势和测序结果一致。此研究为电离辐射应答相关lncRNA的功能研究奠定了基础。","authors":[{"authorName":"李朋飞","id":"84336025-8cdd-48b2-ae73-10581af44d2d","originalAuthorName":"李朋飞"},{"authorName":"胡文涛","id":"d807e8c8-40b2-4afc-b1fd-0f1c38827ea5","originalAuthorName":"胡文涛"},{"authorName":"裴海龙","id":"0b6df3b1-742f-4d8b-b670-4291f17ddcec","originalAuthorName":"裴海龙"},{"authorName":"丁楠","id":"bc413d88-29d9-4579-a4e5-1c6921b8522f","originalAuthorName":"丁楠"},{"authorName":"周光明","id":"2e297502-fdd8-4b65-8e14-60ed09bd8038","originalAuthorName":"周光明"}],"doi":"10.11804/NuclPhysRev.32.04.484","fpage":"484","id":"ea9e983c-c871-4197-b35f-041e6b43894b","issue":"4","journal":{"abbrevTitle":"YZHWLPL","coverImgSrc":"journal/img/cover/YZHWLPL.jpg","id":"78","issnPpub":"1007-4627","publisherId":"YZHWLPL","title":"原子核物理评论 "},"keywords":[{"id":"d144e638-3640-49b4-949d-2aa9af51d60a","keyword":"长链非编码RNA","originalKeyword":"长链非编码RNA"},{"id":"e866d690-6fd5-4a83-a9b7-7d3c0f9c1156","keyword":"电离辐射","originalKeyword":"电离辐射"},{"id":"ebc2601f-c833-4307-ac34-752d41f83015","keyword":"RNA测序","originalKeyword":"RNA测序"},{"id":"21fa1b82-d366-43ba-a6a6-3fc628f1da76","keyword":"实时荧光定量PCR","originalKeyword":"实时荧光定量PCR"}],"language":"zh","publisherId":"yzhwlpl201504019","title":"电离辐射改变长链非编码RNA的表达谱","volume":"32","year":"2015"},{"abstractinfo":"根据10?080份基因组DNA测序的结果，讨论了毛细管阵列电泳测序方法的技术特点，并对影响测序结果的一些因素进行了分析。在此基础上与平板凝胶电泳方法进行比较，显示了毛细管阵列电泳的优点。同时也对大规模测序技术环节之间的协调进行了探讨。","authors":[{"authorName":"甄志成","id":"d31884af-b654-409b-88a9-4f3a888b1db9","originalAuthorName":"甄志成"},{"authorName":"姚志建","id":"99315357-4169-4ece-8823-0a7bf981e9bf","originalAuthorName":"姚志建"}],"doi":"10.3321/j.issn:1000-8713.2001.04.022","fpage":"361","id":"95d6aff7-da4e-4752-ba66-8b3681c47442","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"6f39eb21-3c1e-4fa0-ba8f-a6ef4cd13f45","keyword":"毛细管阵列电泳","originalKeyword":"毛细管阵列电泳"},{"id":"fa89554e-a86b-4f61-bdb8-263b3366b7f1","keyword":"DNA测序","originalKeyword":"DNA测序"},{"id":"347c87a6-9c53-4a8f-a13d-4f31d725ec94","keyword":"基因组","originalKeyword":"基因组"}],"language":"zh","publisherId":"sp200104022","title":"毛细管阵列电泳与规模化DNA测序","volume":"19","year":"2001"},{"abstractinfo":"将二甲基二烯丙基氯化铵(DADMAC)与乙烯吡咯烷酮(VP)共聚得到阳离子型的聚乙烯吡咯烷酮(CPVP),并分别合成了聚二甲基二烯丙基氯化铵(PDADMAC)和聚乙烯吡咯烷酮(PVP)均聚物,用粘度法测定了三者的粘均相对分子质量,用红外光谱表征了其结构.将合成的CPVP与核糖核酸(RNA)以不同比例混合,研究CPVP负载RNA的能力,并与PDADMAC及PVP的负载性能做比较.结果发现,与PDADMAC及PVP相比,当CPVP与RNA按质量比2∶1混合时,形成的复合体纳米粒的平均粒径约为300 nm,分散度指数为0.0966,粒径分布较窄.","authors":[{"authorName":"林媚","id":"885f54fd-7e9d-4e28-ba6d-25678c2ef896","originalAuthorName":"林媚"},{"authorName":"林美玉","id":"c2949613-3126-44e1-b96f-a042ab5b7288","originalAuthorName":"林美玉"},{"authorName":"朱炜","id":"02dc8159-019d-4b07-aea1-6429d4bc3d1f","originalAuthorName":"朱炜"},{"authorName":"李超萍","id":"6150607e-8d18-411f-b834-a2b4b66dcfe6","originalAuthorName":"李超萍"},{"authorName":"陈何璧","id":"548057ea-2d7f-4e46-90d9-19b794ee5158","originalAuthorName":"陈何璧"},{"authorName":"高凡","id":"79e5a72b-4d61-4bb7-9aed-56f88e914695","originalAuthorName":"高凡"},{"authorName":"吴珊月","id":"7ba7f2ab-d4ef-4a19-8439-db3385d66729","originalAuthorName":"吴珊月"},{"authorName":"黄毅楦","id":"8505804d-625f-4b0f-9c8a-84d22dc2594e","originalAuthorName":"黄毅楦"}],"doi":"10.3724/SP.J.1095.2014.30421","fpage":"649","id":"aeca265d-3929-4270-a008-a71e82814ec8","issue":"6","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"c8c2e46a-58ce-4e15-9e9a-84c20191c28c","keyword":"阳离子","originalKeyword":"阳离子"},{"id":"812540a5-540e-4c44-965a-91391ae4bc6e","keyword":"聚乙烯吡咯烷酮","originalKeyword":"聚乙烯吡咯烷酮"},{"id":"52d85371-da25-44da-8e28-874ccf79c9e1","keyword":"核糖核酸","originalKeyword":"核糖核酸"},{"id":"e1c17cdc-2d1f-4ae2-ac6b-e819cbe662db","keyword":"纳米粒子","originalKeyword":"纳米粒子"}],"language":"zh","publisherId":"yyhx201406003","title":"阳离子型聚乙烯吡咯烷酮的制备及其负载RNA性能","volume":"31","year":"2014"},{"abstractinfo":"运用新型的以生物热力学为基础的快速有效的竞争平衡透析法,紫外热力学变温法,圆二色谱法以及荧光光谱等系列方法与技术研究了coralyne与多种核酸的相互识别作用.结果表明,coralyne对polyA-(polyU)2三股螺旋RNA具有选择性并能稳定其结构, 表观结合常数Kapp＝(2.0±0.4)×106 (mol/L)-1,在本实验条件下使其解旋温度提高将近11 ℃.因此,Coralyne是对polyA(polyU)2最具选择性的分子之一.","authors":[{"authorName":"邢菲菲","id":"4c87f960-d5c5-4822-a47d-6348e8df2707","originalAuthorName":"邢菲菲"},{"authorName":"宋广涛","id":"c512f574-2ebc-4bdf-904d-6d267f5a6bd4","originalAuthorName":"宋广涛"},{"authorName":"任劲松","id":"c92d2da9-c9c9-4472-8601-0db6dd24348e","originalAuthorName":"任劲松"}],"doi":"10.3969/j.issn.1000-0518.2006.12.022","fpage":"1404","id":"4cc5e321-248b-46d1-b105-30b47fa0f422","issue":"12","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"b11017ec-5185-4032-9b62-0343d72d13c6","keyword":"三股螺旋RNA","originalKeyword":"三股螺旋RNA"},{"id":"61212daa-e41a-40a4-9dcf-8537232e894f","keyword":"抗癌药物","originalKeyword":"抗癌药物"},{"id":"d15a7e45-231b-475f-8de2-bc2496b90e67","keyword":"稳定","originalKeyword":"稳定"},{"id":"77b86604-c1ff-41f0-a84d-b36ab4bd8278","keyword":"coralyne","originalKeyword":"coralyne"},{"id":"b37d6560-5e65-4d9e-8cb3-c9ef60be8e27","keyword":"识别","originalKeyword":"识别"}],"language":"zh","publisherId":"yyhx200612022","title":"Coralyne对三股螺旋RNA的识别及稳定作用","volume":"23","year":"2006"},{"abstractinfo":"通过高温干烤和焦碳酸二乙酯(DEPC)两种技术除去核糖核酸(RNA)酶，在保证RNA的稳定性的情况下，在1．0%T,0%C的线性聚丙烯酰胺(即丙烯酰胺和双丙烯酰胺的总质量分数是1．0%，交联度为0%)筛分介质中，利用无胶筛分毛细管电泳技术对水稻中的总RNA进行了分析，整个分析过程在15 min内完成。利用5．0%T,0%C线性聚丙烯酰胺筛分介质，对水稻中的转移RNA(tRNA)进行了较为详细的分析，在30 min内可将tRNA分为两组共9个峰。该方法可用于植物中RNA的分析，具有快速、准确的特点。","authors":[{"authorName":"樊兴君","id":"2642f8fa-8cc8-4202-896b-a8cab6fdc389","originalAuthorName":"樊兴君"},{"authorName":"江舸","id":"582678dc-3604-4d05-b62d-eb8ad6c5c458","originalAuthorName":"江舸"},{"authorName":"金由辛","id":"54b6a226-3562-4ed4-b1d7-fd9e36f5159b","originalAuthorName":"金由辛"},{"authorName":"王德宝","id":"5d56e3dc-ca88-4cc3-8d55-ffa0eed5b62e","originalAuthorName":"王德宝"}],"doi":"10.3321/j.issn:1000-8713.2001.02.020","fpage":"167","id":"1052617b-162e-4094-8649-930f9170311f","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"b8394fe7-6cad-474e-b1d0-b0ee871313c8","keyword":"毛细管电泳","originalKeyword":"毛细管电泳"},{"id":"83e8a000-e56d-43aa-ab67-8ab684184310","keyword":"水稻总RNA","originalKeyword":"水稻总RNA"},{"id":"cf140084-1937-4a70-a766-c36271ed87c3","keyword":"水稻转移RNA","originalKeyword":"水稻转移RNA"}],"language":"zh","publisherId":"sp200102020","title":"毛细管电泳技术分析水稻中的总核糖核酸","volume":"19","year":"2001"},{"abstractinfo":"纳米孔单分子分析技术是通过不同的压力驱动分子通过纳米孔,监测分子易位时产生的电流变化.该技术快速,无需标记、扩增等方法,可以检测分析分子性质.随之发展起来的纳米孔测序技术可以将人类基因组测序成本控制在1000美元之内.主要介绍了用于单分子分析的纳米孔技术的研究进展.","authors":[{"authorName":"于静静","id":"d6f919bf-ac95-4ea8-b959-1e10618acc4a","originalAuthorName":"于静静"},{"authorName":"王磊","id":"539b0820-4cc5-4c6e-88e2-130d61319f30","originalAuthorName":"王磊"},{"authorName":"刘全俊","id":"90ea14d5-1801-4270-9b46-f79f0536663e","originalAuthorName":"刘全俊"}],"doi":"10.11896/j.issn.1005-023X.2015.05.019","fpage":"110","id":"6b9fa986-4288-4fe8-8325-df5877029905","issue":"5","journal":{"abbrevTitle":"CLDB","coverImgSrc":"journal/img/cover/CLDB.jpg","id":"8","issnPpub":"1005-023X","publisherId":"CLDB","title":"材料导报"},"keywords":[{"id":"adf0c798-97b1-4e2c-a63f-47efffd006cf","keyword":"纳米孔","originalKeyword":"纳米孔"},{"id":"2af2eed9-b9b6-4621-b279-9a7379395a91","keyword":"单分子","originalKeyword":"单分子"},{"id":"86035213-8117-4e67-8414-99b1da138fca","keyword":"测序","originalKeyword":"测序"},{"id":"3d9bbdea-3b55-42e1-81b8-836591ad955b","keyword":"易位","originalKeyword":"易位"}],"language":"zh","publisherId":"cldb201505019","title":"纳米孔单分子分析技术研究进展","volume":"29","year":"2015"},{"abstractinfo":"MicroRNA(miRNA)是一类大小为19-24个核苷酸的非编码RNA,它们通过与靶基因的信使RNA(mRNA)结合在转录后水平调节靶基因的表达,因而在广泛的生物学过程中发挥重要作用.首先从miRNA的获得和鉴定两方面对目前miRNA发现工作进行了概括,然后分析和总结了近年来人源miRNA发现工作的现状,最后提出有关新miRNA发现工作的新想法.","authors":[],"doi":"","fpage":"172","id":"bb64a624-b038-4eb1-bdce-b492eec2b967","issue":"2","journal":{"abbrevTitle":"YZHWLPL","coverImgSrc":"journal/img/cover/YZHWLPL.jpg","id":"78","issnPpub":"1007-4627","publisherId":"YZHWLPL","title":"原子核物理评论 "},"keywords":[{"id":"a018db7b-0334-4765-a198-828f879484ea","keyword":"microRNA(miRNA)","originalKeyword":"microRNA(miRNA)"},{"id":"103285f9-9152-4834-a88b-58c92311b90b","keyword":"新miRNA发现","originalKeyword":"新miRNA发现"},{"id":"8c5707a6-3337-4d88-a38f-52dc87acadd3","keyword":"重离子辐照","originalKeyword":"重离子辐照"}],"language":"zh","publisherId":"yzhwlpl200902017","title":"MicroRNA发现的研究现状及重离子辐照相关应用前景","volume":"26","year":"2009"},{"abstractinfo":"综述了单纳米孔传感器中的生物纳米孔和固态纳米孔的研究现状,重点介绍了α-HL溶血素孔、生物纳米孔及人工固态纳米孔传感器的制备,以及单纳米孔传感器在单分子检测和DNA测序方面的应用进展,并展望了该领域的发展趋势和应用前景.","authors":[{"authorName":"战胜鑫","id":"55bade84-dbb9-4dbd-80a0-d65199f89063","originalAuthorName":"战胜鑫"},{"authorName":"张晓辉","id":"17dac37e-8b9c-4c5d-bc07-0de2f86aefc4","originalAuthorName":"张晓辉"},{"authorName":"王娜","id":"7a04ad7c-d5cb-4d5b-b75c-43ba750c1b2c","originalAuthorName":"王娜"},{"authorName":"杨晓蕊","id":"2e7978d1-cadd-41a1-8a53-f311f060eca2","originalAuthorName":"杨晓蕊"},{"authorName":"郭明","id":"44423aeb-058d-4bd7-ac50-6d023d634188","originalAuthorName":"郭明"}],"doi":"","fpage":"13","id":"27b1c856-6b32-47ff-8a7a-61ef0f2fa938","issue":"19","journal":{"abbrevTitle":"CLDB","coverImgSrc":"journal/img/cover/CLDB.jpg","id":"8","issnPpub":"1005-023X","publisherId":"CLDB","title":"材料导报"},"keywords":[{"id":"8e971f75-9142-43b1-9139-ffedbe40462a","keyword":"纳米孔","originalKeyword":"纳米孔"},{"id":"441b995b-b061-43e9-94dd-6d88b10f2909","keyword":"α-HL溶血素","originalKeyword":"α-HL溶血素"},{"id":"a2dde179-3b97-4b36-a967-f3423dcaacad","keyword":"膜片钳","originalKeyword":"膜片钳"},{"id":"18132cff-45b2-476e-b6a9-0366c579d069","keyword":"单分子检测","originalKeyword":"单分子检测"},{"id":"3083070a-34fe-46d5-b961-4e01ab7e2d0c","keyword":"单纳米孔传感器","originalKeyword":"单纳米孔传感器"}],"language":"zh","publisherId":"cldb201219004","title":"单纳米孔传感器分析研究进展","volume":"26","year":"2012"},{"abstractinfo":"对黄金价格建立了ARMA-马尔科夫预测模型,该模型将数据统计特征与灰色理论密切结合.ARMA部分用来揭示预测序列的线性变化趋势,而马尔科夫状态转移概率矩阵用来确定状态转移的规律.实证研究表明,该模型预测精度优于ARMA模型以及灰色马尔科夫模型的预测精度.","authors":[{"authorName":"张延利","id":"ba8b9ad4-b52b-422f-b79c-6a3e0329faa7","originalAuthorName":"张延利"}],"doi":"","fpage":"6","id":"f1e47c16-6228-4eaa-8682-4cb4497edd51","issue":"9","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"0e286fb2-ab77-4169-9146-ea188673c707","keyword":"黄金价格","originalKeyword":"黄金价格"},{"id":"eff1541a-0110-46a4-9dfd-2c2d61fb7625","keyword":"ARMA模型","originalKeyword":"ARMA模型"},{"id":"4cb5286b-ebfc-47ee-8873-12de18b17d7a","keyword":"灰色马尔科夫模型","originalKeyword":"灰色马尔科夫模型"},{"id":"abc457b5-f04b-4091-9cb6-667724497c5b","keyword":"ARMA-马尔科夫模型","originalKeyword":"ARMA-马尔科夫模型"}],"language":"zh","publisherId":"huangj201209002","title":"新马尔科夫模型的黄金价格短期预测","volume":"33","year":"2012"},{"abstractinfo":"对微流控芯片实验室在基因分析中应用研究的最新进展予以综述,特别注意到了这种新技术平台在不同类型的基因多态性检测和脱氧核糖核酸(DNA)测序中的贡献,在一定程度上反映了这种贡献在临床诊断、法医学鉴定等领域已经产生的影响.","authors":[{"authorName":"秦建华","id":"d7e9e953-34bc-48ee-92d3-0a12a14c2f85","originalAuthorName":"秦建华"},{"authorName":"冯应升","id":"e698197d-b77e-42c3-83d1-909d7210bd00","originalAuthorName":"冯应升"},{"authorName":"林炳承","id":"87114c84-b411-4ba7-8dd2-102ba15bb5e0","originalAuthorName":"林炳承"}],"doi":"10.3321/j.issn:1000-8713.2003.05.006","fpage":"464","id":"d3a8e092-45f8-4624-ad35-d4435ca5a2c3","issue":"5","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 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