在电子储存环ELISA上研究了气相生物分子的光吸收. 通过研究发现, 储存时间长并具有合适的粒子探测系统的储存环是进行成色基团光物理研究的理想工具. 讨论了绿色荧光蛋白质(GFP)的光吸收特性, 并将气相光吸收测量谱与液体中蛋白质和成色基团的吸收形貌进行了比较, 认为GFP的吸收特性可以归因于成色基团的固有化学性质.
Absorption of gas-phase biomolecules has been studied at the electrostatic ion storage ring ELISA. It is demonstrated that the storage ring technique with long storage times and suitable particle detection is ideal for studies of the photo-physics of chromophores. Here we discuss the absorption characteristics of the chromophores of the Green Fluorescent Protein (GFP) and compare our gas-phase absorption measurements with absorption profiles of the protein and chromophores in liquids. It is argued that the absorption characteristics of GFP are mainly ascribed to intrinsic chemical properties of the chromophore.
参考文献
| [1] | Mller.S P.ELISA,an Electrostatic Storage Ring for Atomic Physics[J].Nucl Instr and Meth (in Physics Research),1997,A394:281. |
| [2] | Tsien R Y.The Green Fluorescent Protein[J].Annu Rev Biochem,1998,67:509. |
| [3] | Cubitt A B,Heim R,Adams S R,et al.Understanding,Improving and Using Green Fluorescent Proteins[J].Trends Biochem Sci,1995,20:448. |
| [4] | Prasher D C.Using GFP to See the Light[J].Trends Genet,1995,11:320. |
| [5] | Gerdes H H,Kaethe C.Green Fluorescent Protein:Applications in cell biology[J].FEBS Lett,1996,389:44. |
| [6] | Webber N M,Litvinenko K L,Meech S R.Radiationless Relaxation in a Synthetic Analogue of the Green Fluorescent Protein Chromophore[J].J Phys Chem,2001,B105:8 036. |
| [7] | Andersen J U,et al.Submitted to Review of Sci Instruments (to be published). |
| [8] | Lapierre A,Nielsen S B,Nielsen I B,et al.J Phys Chem,2002,(to be published). |
| [9] | Niesen S B,Lapierre A,Andersen J U,et al.Absorption Spectrum of the Green Fluorescent Protein Chromophore Anion In Vacuo[J].Phys Rev Lett,2001,87:2281021. |
| [10] | Orm M,Cubitt A B,Kallio K,et al.Crystal Structure of the Aequorea Victoria Green Fluorescent Protein[J].Science,1996,273:1 392. |
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