柞蚕丝素具有优良的生物相容性、低的生物免疫反应、细胞亲和性等特性,是一种性能优越的潜在生物医学材料。本文以我国特有的柞蚕丝为原料,以水作为溶剂,通过静电纺丝技术制备了柞蚕丝素纳米纤维。并利用SEM、XRD、DSC、核磁共振等测试手段研究了柞蚕丝素水溶液静电纺纳米纤维的形貌、构象、结晶结构等。研究表明：柞蚕丝素水溶液静电纺丝的最佳浓度为29%～31%（w/w）、所得纤维的直径范围在217～717nm之间,构象以无规卷曲和α-螺旋为主,水蒸气处理后,纤维构象由α-螺旋向β-折叠转变。
Tussah silk fibroin（TSF） is one of the excellent potential biomedical materials due to its biological compatibility,low biology immune response,and cell affinity.Electrospun nanofibers have been acquired using the TSF as raw material and water as solvent.The morphology,conformation and crystal structure of the nanofibers were investigated using scanning electron microscopy（SEM）,X-ray diffractometry（XRD）,differential scanning calorimetry（DSC） and nuclear magnetic resonance（NMR） spectroscopy.The optimal mass concentration of electrospinning TSF aqueous solution was 29%～31%.The diameter of the fibers ranged from 217nm to 717nm.The structure of TSF nanofibers was random coil and α-helix,but after post treatment with water vapor,the α-helix structure of the nanofibers changed into β-sheet.
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