以脐血来源的间充质干细胞(UCB-MSC)为研究对象,应用MTT方法观测壳寡糖对细胞增殖的影响,荧光定量PCR技术研究壳寡糖对细胞周期蛋白cyclinD1和骨特异转录因子RUNX2mRNA表达的调控.结果显示壳寡糖还可促进脐血间充质干细胞增殖,且浓度为300μg/ml的壳寡糖作用最为显著.同时荧光定量PCR结果说明壳寡糖上调cyclin D1和RUNX2基因表达,即一方面壳寡糖通过提高cyclin D1的水平促进脐血间充质干细胞的增殖,另一方面其可通过上调RUNX2的表达促进细胞向成骨细胞分化.
参考文献
| [1] | Schneider RK;Puellen A;Kramann .The osteogenic differentiation of adult bone marrow and perinatal umbilical mesenchymal stem cells and matrix remodelling in three-dimensional collagen scaffolds.[J].Biomaterials,2010(3):467-480. |
| [2] | Hildebrandt C;Buth H;Thielecke H .Influence of cell culture media conditions on the osteogenic differentiation of cord blood-derived mesenchymal stem cells.[J].Annals of anatomy =: Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft,2009(1):23-32. |
| [3] | 金琪琳,黄云超.生物材料对细胞生物学行为的影响[J].材料科学与工程学报,2007(02):318-320,324. |
| [4] | Yang Y;Liu M;Gu Y;Lin S;Ding F;Gu X .Effect of chitooligosaccharide on neuronal differentiation of PC-12 cells.[J].Cell biology international,2009(3):352-356. |
| [5] | Jung WK;Moon SH;Kim SK .Effect of chitooligosaccharides on calcium bioavailability and bone strength in ovariectomized rats.[J].Life sciences,2006(9):970-976. |
| [6] | Juthamas Ratanavaraporn;Sorada Kanokpanont;Yasuhiko Tabata .Growth and osteogenic differentiation of adipose-derived and bone marrow-derived stem cells on chitosan and chitooligosaccharide films[J].Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides,2009(4):873-878. |
| [7] | 孙奋勇,汪炬,孙晋华,戴云,邱垂源,洪岸.重组人骨形成蛋白-2在大肠杆菌中的表达、纯化和复性[J].中国病理生理杂志,2005(08):1480-1485. |
| [8] | Fu M;Wang C;Li Z;Sakamaki T;Pestell RG .Minireview: Cyclin D1: normal and abnormal functions.[J].Endocrinology,2004(12):5439-5447. |
| [9] | Klein EA;Assoian RK .Transcriptional regulation of the cyclin D1 gene at a glance.[J].Journal of Cell Science,2008(23):3853-3857. |
| [10] | Jonason JH;Xiao G;Zhang M .Post-translational Regulation of Runx2 in Bone and Cartilage.[J].Journal of Dental Research: Official Publication of the International Association for Dental Research,2009(8):693-703. |
| [11] | Niranjan Rajapakse;Se-Kwon Kim .Enzymatic production and biological activities of chitosan oligosaccharides (COS):A review[J].Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides,2005(4):357-368. |
| [12] | Wen-Peng Yuan,Bing Liu,Chang-Heng Liu,Xiao-Jun Wang,Mian-Song Zhang,Xiu-Mei Meng,Xue-Kui Xia.Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats[J].世界胃肠病学杂志（英文版）,2009(11):1339-1345. |
| [13] | 张伟,沈若武,王守彪.壳寡糖对骨折成骨细胞内骨形态发生蛋白的影响[J].齐鲁医学杂志,2005(03):247-248,250. |
| [14] | 万丽卿,胡富强,袁弘.A549细胞对壳寡糖及其纳米粒的摄取作用[J].药学学报,2004(03):227-231. |
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