用静电纺丝技术制备了再生桑蚕丝素纳米纤维,并用1-（3-二甲基氨基丙基）-3-乙基碳化二亚胺（EDC）和N-羟基丁二酰亚胺（NHS）进行交联改性,考察了交联改性前后,桑蚕丝素纳米纤维微观形貌及聚集态结构等的变化,采用扫描电镜（SEM）、X射线衍射（XRD）及红外光谱（FT-IR）等测试方法对纳米纤维进行表征。研究结果表明,经EDC/NHS交联改性后,纤维直径由250 nm增加到320 nm,纤维表面变粗糙,且纤维发生弯曲变形;丝素的结构以Silk II为主,并含有部分无规卷曲或Silk I构象;桑蚕丝纳米纤维的力学性能和亲水性有所提高,且交联改性后的纳米纤维具有良好的细胞相容性。
Regenerated silk fibroin nanofiber prepared by electrospinning methods has crosslinked with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide/N-hydroxy succinimide(EDC/NHS).The morphology of uncrosslinked and crosslinked silk fibroin nanofiber mats was studied by SEM,and its aggregated structure was characterized by X-ray diffraction and FT-IR analysis et al.The results show that after crosslinking,the average diameter of crosslinked nanofiber increases from 250 nm to 320 nm;the crosslinked silk fibroin nanofiber deforms and its surface becomes rough;the porosity of silk fibroin nanofiber mats decreases.At the same time,the secondary structure of crosslinked silk fibroin nanofiber is mainly in Silk II,containing partial random coil or Silk I conformation.The crosslinked silk fibroin nanofiber has better mechanical properties and hydrophilicity than the uncrosslinked materials.In addition,the crosslinked silk fibroin nanofiber shows good biocompatibility.
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