以透明质酸钠浓度、交联剂投入量、反应温度、溶胀时间作为考察因素,抗酶系数作为评价指标,采用L9(34)正交设计表优化交联透明质酸钠支架材料制备工艺参数并建立支架的体外抗酶降解性测定方法.正交试验结果经直观和方差分析显示,透明质酸钠浓度、交联剂投入量具有显著性影响,在透明质酸钠浓度为5％,交联剂投入量为5％,反应温度40℃和溶胀时间4h的条件下,可以制得体外抗酶降解性较强的交联透明质酸钠支架材料,其可作为一种细胞支架用于软骨组织工程研究.
参考文献
| [1] | Laurent T C;Fraser J R .Hyalurona[J].The FASEB Journal,1992,6(07):397. |
| [2] | Hoffman AS .Hydrogels for biomedical applications.[J].Advanced drug delivery reviews,2002(1):3-12. |
| [3] | Responte D J;Natoli R M;Athanasiou K A .Identification of potential biophysical and molecular signalling mechanisms underlying hyaluronic acid enhancement of cartilage formation[J].Journal of the Royal Society Interface,2012,9(77):3564. |
| [4] | Narins RS;Bowman PH .Injectable skin fillers.[J].Clinics in plastic surgery,2005(2):151-162. |
| [5] | Dover J S;Rubin M G;Bhatia A C .Review of the efficacy,durability and safety data of two nonanimal stabilized hyaluronic acid fillers from a prospective,randomized,comparative,multicenter study[J].DERMATOLOGIC SURGERY,2009,35(z1):22. |
| [6] | Cianflocco A J .Viscosupplementation in patients with osteoarthritis of the knee[J].Postgraduate Medicine,2013,125(01):97. |
| [7] | Bitter T;Muir H M .A modified uronic acid carbarbazole reation[J].Analytical Biochemistry,1962,4:330. |
| [8] | 贺艳丽,陈建英,王玉玲.交联透明质酸体外耐酶试验方法的研究[J].食品与药品,2005(01):32-35. |
| [9] | 贺艳丽,陈建英,刘杰,王玉玲,娄红祥.交联透明质酸膜的特性与生物相容性的初步研究[J].中国生化药物杂志,2006(01):25-28. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%