在丙交酯与己内酯的共聚物PLCL中加入明胶(Gelatin,Gel)或胶原(Collagen,Col),并调节其含量比率,用静电纺丝法制备成纳米纤维膜.测得这些膜的纤维平均直径在(750±390nm)到(1670±260nm),接触角测量结果显示明胶或胶原的含量与膜表面的亲水性正相关.在上述不同组分的纤维膜支架上培养小鼠颅骨前成骨细胞(MC3T3-E1),研究了各种膜对细胞的增殖和矿化的影响.
参考文献
| [1] | So Eun Lee;Youngmee Jung;Soo Hyun Kim;Sang-Heon Kim;Jong Won Rhie;Young Ha Kim;Byoung Goo Min .CARTILAGINOUS TISSUE FORMATION WITH AN ELASTIC PLCL SCAFFOLD AND HUMAN ADIPOSE TISSUE-DERIVED STROMAL CELLS[J].Key engineering materials,2007(342/343):385-388. |
| [2] | Kikuchi M;Koyama Y;Yamada T;Imamura Y;Okada T;Shirahama N;Akita K;Takakuda K;Tanaka J .Development of guided bone regeneration membrane composed of (Formula: see text) -tricalcium phosphate and poly (l-lactide-co-glycolide-co- (Formula: see text) -caprolactone) composites.[J].Biomaterials,2004(28):5979-5986. |
| [3] | 李沁华,莫小慧,杜文旭.聚乙烯醇-透明质酸-胶原组织工程支架研究[J].暨南大学学报(自然科学与医学版),2009(03):319-324. |
| [4] | 秦胜男,陈鸿辉.胶原在软骨组织工程中的应用[J].中国矫形外科杂志,2009(13):998-1000. |
| [5] | Clarke K I;Graves S E;Wong A T C et al.Investigation into the formation and mechanical properties of a bioactive material based on collagen andcalcium phosphate[J].Journal of Materials Science:Materials in Medicine,1993,4:107. |
| [6] | 廖鹰扬,陈鸿辉,杨小红,梁佩红,戴丽冰,谭见容.Ⅱ型胶原海绵的材料特性及对软骨缺损的影响[J].中国矫形外科杂志,2009(13):1024-1028. |
| [7] | 柳晖,李新强,陈武鹏,向红霞,胡检,张显文.转染血管紧张素Ⅰ骨髓间充质干细胞复合胶原构建组织工程皮肤修复烫伤后的创面[J].中国组织工程研究与临床康复,2009(15):2859-2864. |
| [8] | 陈晓东,江琼,吴伯瑜,王顺宾.聚乳酸-羟基乙酸共聚物和胶原膜对体外培养人表皮角质细胞分泌细胞因子的影响[J].福建医科大学学报,2009(01):5-9. |
| [9] | Tan AR;Ifkovits JL;Baker BM;Brey DM;Mauck RL;Burdick JA .Electrospinning of photocrosslinked and degradable fibrous scaffolds.[J].Journal of biomedical materials research, Part A,2008(4):1034-1043. |
| [10] | Chen F;Li XQ;Mo XM;He CL;Wang HS;Ikada Y .Electrospun chitosan-P(LLA-CL) nanofibers for biomimetic extracellular matrix[J].Journal of biomaterials science,2008(5):677-691. |
| [11] | J. Lannutti;D. Reneker;T. Ma;D. Tomasko;D. Farson .Electrospinning for tissue engineering scaffolds[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2007(3):504-509. |
| [12] | Chapekar M S .Tissue engineering:Challenges and opportunities[J].Journal of Biomedical Materials Research,2000,53:617. |
| [13] | Li W J;Laurencin C T;Caterson E J et al.Electrospun nanofibrous structure:a novel scaffold for tissue engineering[J].Journal of Biomedical Materials Research,2002,60:613. |
| [14] | Venugopal J;Zhang Y Z;Ramakrishna S .In vitro cuhure of human dermal fibroblasts on electrospun polycaprolactone collagen nanofibrous membrane[J].Artificial Organs,2006,30:438. |
| [15] | Yanzhong Zhang;Hongwei Ouyang;Chwee Teck Lim .Electrospinning of Gelatin Fibers and Gelatin/PCL Composite Fibrous Scaffolds[J].Journal of biomedical materials research, Part A,2005(1):156-165. |
| [16] | Badami AS;Kreke MR;Thompson MS;Riffle JS;Goldstein AS .Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates[J].Biomaterials,2006(4):596-606. |
| [17] | Jayarama Reddy Venugopal;Sharon Low;Aw Tar Choon et al.Nanobioengineered electrospun composite nanofibers and ostcoblasts for bone regeneration[J].Artificial Organs,2008,32(05):388. |
| [18] | Teixeira A I;Nealey P F;Murphy C J .Responses of human keratocytes to micro and nanostructured substrates[J].Journal of Biomedical Materials Research Part A,2004,71:369. |
| [19] | Bong Geun Chung;Lisa A.Flanagan;Seog Woo Rhee;Philip H.Schwartz;Abraham P.Lee;Edwin S.Monuki;Noo Li Jeon .Human neural stem cell growth and differentiation in a gradient-generating microfluidic device[J].Lab on a chip,2005(4):401-406. |
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