以二氯硅（IV）酞菁（SiPcCl2）为原料,与2-异丙基-6-甲基-4-嘧啶醇（PyO）反应合成轴向取代二-（2-异丙基-6-甲基-4-嘧啶氧基）硅（IV）酞菁配合物SiPc（PyO）2,并采用元素分析、红外光谱（IR）、核磁共振（1H-NMR）和质谱（MS）对其结构进行了表征。以两亲性嵌段共聚物聚赖氨酸-聚乙二醇-聚赖氨酸（PLL-PEG-PLL）为载体,透析法制备负载SiPc（PyO）2的聚合物纳米粒子SiPc（PyO）2/m。SiPc（PyO）2/m呈球形,直径约35 nm。SiPc（PyO）2包裹入聚合物胶束后,SiPc（PyO）2/m的Q带最大吸收峰强度降低,位置蓝移,荧光强度增加,荧光寿命增长。不同孵育时间HUVEC细胞的SiPc（PyO）2和SiPc（PyO）2/m的药代动力学表明,与自由酞菁3相比,SiPc（PyO）2/m摄取率增加,而且提前1 h达到最大浓度。
A axial substituted di-（2-isopropy1-6-methyl-4-pyrimidyloxy） silicon（Ⅳ） phthalocyanine（SiPc（PyO）2） was synthesized by reaction of dichloride silicon（Ⅳ） phthalocyanine with 2-isopropy1-6-methyl-4-pyrimidylol.The structure of（SiPc（PyO）2） was characterized by IR,1H-NMR,electrospray ionization-mass spectrometry（ESI-MS）,UV/Vis,fluorescence spectroscopy and elemental analysis.The polyion complex micelle SiPc（PyO）2/m was prepared using amphiphilic triblock copolymer poly（L-lysine）-poly（ethylene glycol）-poly（L-lysine） as carrier by dialyze method.The morphology and photophysical properties of SiPc（PyO）2/m were characterized by AFM,UV/Vis and fluorescent spectroscopy.The time-dependence uptake amount of SiPc（PyO）2 and SiPc（PyO）2/m in human umbilical vein endothelial cells（HUVEC） cells showed that the uptake amount of SiPc（PyO）2/m was significantly increased compared with SiPc（PyO）2.Therefore,SiPc（PyO）2/m would be a potential photosensitizer and may have a high utility for in vivo photodynaime therapy（PDT） of choroidal neovascularization.
参考文献
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