对含硅羟基磷灰石(Si-HA)与人血清白蛋白(HSA)的吸附特性及动力学特征进行了研究,计算了表观活化能,用等温吸附曲线进行拟合.结果表明,该吸附属于Langmuir型.Si-HA和HA吸附HSA的表观活化能分别为21.28和35.57 kJ·mol-1,20℃下吸附反应的速率常数分别为1.48和1.34 min-1,饱和吸附量分别为25.34和21.34 mg·g-1,Si-HA具有比纯HA更好的吸附优势.通过XRD、FTIR及荧光光谱分析,探讨了HSA在Si-HA和HA表面的吸附作用机制,表明Si-HA与蛋白表面吸附反应是以化学吸附为主、包含物理吸附的混合吸附过程.从分子结构的角度揭示了Si-HA和HA吸附蛋白质性能的差异,为进一步探讨Si-HA和HA生物相容性提供了新途径.
参考文献
| [1] | Suchanek W,Yoshimura M.Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants[J].J Mater Rees,1998,13(1):94-117. |
| [2] | Xiao L T,Xiu F X,Rong F L.Structural characterization of silicon-substituted hydroxyapatite synthesized by a hydrothermal method[J].Materials Letters,2005,59 (10):3841 -3846. |
| [3] | Thian E S,Huang J,Best S M,Barber Z H.Siliconsubstituted hydroxyapatite:The next generation of bioactive coatings[J].Materials Science and Engineering C,2007,27(2):251-256. |
| [4] | Suzuki K,Yumura T,Mizuguchi M0 et al.Apatite-silica gel composite materials prepared by a new alternate soaking process[J].J Sol-Gel Sci Technol,2001,21(1):55-63. |
| [5] | 吕彩霞,姚子华.纳米羟基磷灰石/壳聚糖-硫酸软骨素复合材料的制备及其性能研究[J].复合材料学报,2007,24(1):110-115.Lu Caixia,Yap Zihua.Preparation and characterization of nano-hydroxyapatite/chitosan-chondroitin sulfate composite materials[J].Acta Materiae Compositae Sinica,2007,24(1):110-115. |
| [6] | 王振林,闰玉华,万涛.羟基磷灰石/胶原类骨仿生复合材料的制备及表征[J].复合材料学报,2005,22(2):83-86.Wang Zhenlin,Yan Yuhua,Wan Tap.Preparation and characterization of hydroxyapatite/collagen bone-like biomimetic composite[J].Acta Materiae Compositae Sinica,2005,22(2):83-86. |
| [7] | Yap S H,Edward C,Hok S L Growth of phosphate coating on titanium substrate by hydrothermal process[J].Ceramics International,1998,24(1):7-12. |
| [8] | 全仁夫,杨迪生,吴晓春.二氧化锆梯度复合羟基磷灰石生物材料的制备及其生物相容性[J].复合材料学报,2006,23(3):117-123.Quan Renfu,Yang Disheng,Wu Xiaochun.Preparation and biocompatibility of graded zirconia-hydroxyapatite composite bioceramic[J].Acta Materiae Compositae Sinica,2006,23(3):117-123. |
| [9] | 许凤兰,李玉宝,姚晓明.纳米羟基磷灰石/聚乙烯醇复合人工角膜材料[J].复合材料学报,2005,22(1):27-31.Xu Fenglan,Li Yubao,Yap Xiaoming.Nano hydroxyapatite/polyvinyl alcohol composite for keratoprosithesis[J].Acta Materiae Compositae Sinica,2005,22 (1):27-31. |
| [10] | Gibson I R,Best S M,Bonfield W.Chemical characterization of silicon-substituted hydroxyapatite[J].Biomed Mater Res,1999,4(2):422-428. |
| [11] | Niwa M,Wei L,Sato I,et al.The adsorptive property of hydroxyapatite to albumin dextran and lipids[J].Biomed Mater Eng,1999,9(3):163-169. |
| [12] | Tanizawa Y,Suzuki T.Synthesis of Si,Mg substituted hydroxyapatites and their sinterlng behaviors[J].Phosphorus Res Bull,1994,4(1):83-88. |
| [13] | Mochaies C,Briak B,Abdeslac H E.Obtaining of silicatesubstituted calcium deficient hydroxyapatite by mechanosynthesis[J].Key Eng Mater,2004,254(2):107-110. |
| [14] | 叶青,胡仁,林种玉.羟基磷灰石与牛血清白蛋白相互作用的原位红外光谱研究[J].高等学校化学学报,2006,27(8):1552-1554.Ye Qing,Hu Ren,Lin Zhongyu.In situ ATR-FTIR study on the interaction o[HA with bovine serum albumin[J].Chemical Journal of Chinese Universities,2006,27(8):1552-1554. |
| [15] | 刘永明,李桂芝,宋万坤.盐酸拓扑替康与人血清白蛋白的相互作用及分子模拟[J].物理化学学报,2006,22(12):1456-1459.Liu Yongming,Li Guizhi,Song Wankun.Molecular simulation and interaction between human serum albumin and topotecan hydrochloride[J].Acta Phys Chim Sin,2006,22(12):1456-1459. |
| [16] | Tanrek A,Aoki H I.Medical applications and adsorption property of hydroxyapatite[J].J Mater Sci Mater Med,1999,25(2):51-55. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%