本工作设计研制了一套激光捕获与原位稳态及时间分辨荧光光谱检测系统,该系统将激光捕获技术与稳态及时间分辨荧光光谱检测技术相结合,实现了对亚微米级微粒的原位稳态及时间分辨发光性质研究.利用研制的仪器研究了荧光标记聚苯乙烯微球的激光捕获过程、以及捕获微粒的原位稳态及时间分辨荧光光谱.本工作为单个纳米粒子发光性质的研究开辟了一条新的途径.
参考文献
| [1] | 李银妹.生命科学新技术-光镊原理、技术和应用[M].合肥:中国科学技术大学出版社,1996 |
| [2] | Ashkin A;Dziedzic J M;Bjorkholm J E;Chu S .Observation of a single-beam gradient force optical trap for dielectric particles[J].Optics Letters,1986,11:288-290. |
| [3] | Ashkin A;Dziedzic J M .Optical trapping and manipulation of viruses and bacteria"[J].Science,1987,235:1517-1520. |
| [4] | Kim HB;Hayashi M;Nakatani K;Kitamura N;Sasaki K;Hotta JI .IN SITU MEASUREMENTS OF ION-EXCHANGE PROCESSES IN SINGLE POLYMER PARTICLES - LASER TRAPPING MICROSPECTROSCOPY AND CONFOCAL FLUORESCENCE MICROSPECTROSCOPY[J].Analytical chemistry,1996(3):409-414. |
| [5] | Ashkin A .Acceleration and trapping of particles by radiation pressure[J].Physical Review Letters,1970,24:156-159. |
| [6] | Tanaka Y;Yoshikawa H;Masuhara H .Two-photon fluorescence spectroscopy of individually trapped pseudoisocyanine J-aggregates in aqueous solution[J].Journal of Physical Chemistry B,2006,110:17906-17911. |
| [7] | J. Hofkens;J. Hotta;K. Sasaki .Molecular Association by the Radiation Pressure of a Focused Laser Beam: Fluorescence Characterization of Pyrene-Labeled PNIPAM[J].Journal of the American Chemical Society,1997(11):2741-2742. |
| [8] | Jinping Chen;Shayu Li;Lu Zhang;Baining Liu;Yongbin Han;Guoqiang Yang;Yi Li .Light-Harvesting and Photoisomerization in Benzophenone and Norbornadiene-Labeled Poly(aryl ether) Dendrimers via Intramolecular Triplet Energy Transfer[J].Journal of the American Chemical Society,2005(7):2165-2171. |
| [9] | Masuo S;Yoshikawa H;Asahi T;Masuhara H Sato T Jiang D L Aida T .Fluorescence spectroscopic properties and single aggregate structures of pi-conjugated wire-type dendrimers[J].Journal of Physical Chemistry B,2003,107:2471-2479. |
| [10] | Taguchi K.;Ueno H. .Rotational manipulation of a yeast cell using optical fibres[J].Electronics Letters,1997(14):1249-1250. |
| [11] | Taguchi K.;Ueno H. .Optical trapping of dielectric particle and biological cell using optical fibre[J].Electronics Letters,1997(5):413-414. |
| [12] | Jensen-McMullin C;Lyons E R;Jiang Y;Lee H P .Optical trapping,excitation and detection of polystyrene beads in a fiber-based silicon V-groove system[J].Optical Fibers and Sensors for Medical Applications,2001,253:125-131. |
| [13] | Arai Y;Yasuda R;Akashi K;Harada Y Miyata H Kinosita K Itoh H .Tying a molecular knot with optical tweezers[J].Nature,1999,399:446-448. |
| [14] | Hosokawa C;Yoshikawa H;Masuhara H .Optical assembling dynamics of individual polymer nanospheres investigated by single-particle fluorescence detection[J].Physical review, E. Statistical, nonlinear, and soft matter physics,2004(6 Pt.1):1410-1-1410-7-0. |
| [15] | Shen Y R.The Principles of Nonlinear Optics[M].New York:John Wiley and Sons,Inc,1984 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%