在玻碳电极上修饰一层表面均匀的聚吡咯膜,然后利用钙钛矿和壳聚糖的复合膜来固定牛血红蛋白(Hb),制备出性能良好的过氧化氢生物传感器.采用循环伏安(CV)和扫描电镜对修饰电极进行了表征.该传感器对H2O2具有好的催化响应,且响应快.在优化的实验条件下,所制备的传感器对H2O2的线性范围为7.0×10-6～1.5×10-3mol/L,检测限为9.0×10-8mol/L.
参考文献
| [1] | 罗国安;王宗花;王义明.生物兼容性电极构置及应用[M].北京:科学出版社,2006:5. |
| [2] | Tangkuaram T;Ponchio C;Kangkasomboon T .[J].Biosensors and Bioelectronics,2007,22:2071-2078. |
| [3] | Razola SS.;Ruiz BL.;Diez NM.;Mark HB.;Kauffmann JM. .Hydrogen peroxide sensitive amperometric biosensor based on horseradish peroxidase entrapped in a polypyrrole electrode[J].Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices,2002(11/12):921-928. |
| [4] | Du Pan;Zhou Bo;Cai Chenxin .[J].Journal of Electroanalytical Chemistry,2008,614:149-156. |
| [5] | Zhou Kangfu;Zhu Yihua;Ling Xiao et al.[J].Electrochimica Acta,2010,55:3055-3060. |
| [6] | 万莉 .基于血红蛋白/壳聚糖膜的传感器的研究及应用[D].青岛科技大学,2009. |
| [7] | Korri-Youssoufi, H;Desbenoit, N;Ricoux, R;Mahy, JP;Lecomte, S .Elaboration of a new hydrogen peroxide biosensor using microperoxidase 8 (MP8) immobilized on a polypyrrole coated electrode[J].Materials science & engineering. C, Biomimetic and supramolecular systems,2008(5/6):855-860. |
| [8] | Du Ying;Luo Xiliang;Xu Jingjuan et al.[J].Bioeleetrochemistry,2007,70:342-347. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%