制备壳聚糖/羟基磷灰石-庆大霉素(CS/HA-G)缓释材料,评价其抗茵性能在骨髓炎的治疗中的应用前景;并从微观角度初步探讨药物对大肠杆菌的作用机制.对CS/HA-G缓释材料进行体外缓释行为研究及抗茵实验;并以原子力显微镜(AFM)观察大肠杆茵在药物作用前后表面形态结构的变化.CS/HA-G对大肠杆茵的抑茵效果显著,维持有效释药时间长达30d以上.AFM观察显示药物作用于大肠杆菌后菌体高度和表面平均粗糙度(Ra)均下降,有内容物渗漏.CS/HA是一种理想的庆大霉素载体材料.CS/HA-G的缓释作用和缓释规律显示出该材料在大肠杆菌引发的骨髓炎的防治中具有极大的临床应用潜能.
参考文献
| [1] | Kanellakopoulou K;Giamarellos Bourboulis-EJ .Carrier systems for the local delivery of antibiotics in bone infections.[J].Drugs: International Journal of Current Therapeutics and Applied Pharmacology Reviews, Featuring Evaluations on New Drugs, Review Articles on Drugs and Drug Therapy, and Drug Literature Abstracts,2000(6):1223-1232. |
| [2] | Hoang Q Q;Sicheri F;Howard A J et al.[J].Nature,2003,425(6961):977-980. |
| [3] | Radin S.;Ducheyne P.;Cuckler JM.;Campbell JT. .CALCIUM PHOSPHATE CERAMIC COATINGS AS CARRIERS OF VANCOMYCIN[J].Biomaterials,1997(11):777-782. |
| [4] | Habraken W;Wolke J;Janse J A .[J].Advanced Drug Delivery Reviews,2007,59:234-248. |
| [5] | Stallmann HP;Faber C;Bronckers AL;Nieuw Amerongen AV;Wuisman PI .Osteomyelitis prevention in rabbits using antimicrobial peptide hLF1-11- or gentamicin-containing calcium phosphate cement.[J].The Journal of Antimicrobial Chemotherapy,2004(2):472-476. |
| [6] | Ruszczak Z;Friess W .Collagen as a carrier for on-site delivery of antibacterial drugs.[J].Advanced drug delivery reviews,2003(12):1679-1698. |
| [7] | Joosten U;Joist A;Gosheger G;Liljenqvist U;Brandt B;von Eiff C .Effectiveness of hydroxyapatite-vancomycin bone cement in the treatment of Staphylococcus aureus induced chronic osteomyelitis.[J].Biomaterials,2005(25):5251-5258. |
| [8] | Xu QG;Czemuszka JI .Controlled release of amoxicillin from hydroxyapatite-coated poly(lactic-co-glycolic acid) microspheres[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2008(2):146-153. |
| [9] | Xu QG;Tanaka Y;Czernuszka JT .Encapsulation and release of a hydrophobic drug from hydroxyapatite coated liposomes[J].Biomaterials,2007(16):2687-2694. |
| [10] | Ho ML;Fu YC;Wang GJ;Chen HT;Chang JK;Tsai TH;Wang CK .Controlled release carrier of BSA made by W/O/W emulsion method containing PLGA and hydroxyapatite[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2008(2):142-148. |
| [11] | Shanmuganathan S;Shanumugasundaram N;Adhirajan N et al.[J].Carbohydrate Polymers,2008,73(02):201-211. |
| [12] | Martinoa A D;Sittinger M;Risbuda M V et al.[J].Biomaterials,2005,26:5983-5990. |
| [13] | Kim I Y;Seo S J;Moon H S et al.[J].Biotechnology Advances,2008,26:1-21. |
| [14] | Liu TY;Chen SY;Li JH;Liu DM .Study on drug release behaviour of CDHA/chitosan nanocomposites-Effect of CDHA nanoparticles[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2006(1):88-95. |
| [15] | Liu TY;Liu TY;Chen SY;Chen SC;Liu DM .Effect of hydroxyapatite nanoparticles on ibuprofen release from carboxymethyl-hexanoyl chitosan/O-hexanoyl chitosan hydrogel[J].Journal of nanoscience and nanotechnology,2006(9/10):2929-2935. |
| [16] | Rusu VM;Ng CH;Wilke M;Tiersch B;Fratzl P;Peter MG .Size-controlled hydroxyapatite nanoparticles as self-organized organic-inorganic composite materials.[J].Biomaterials,2005(26):5414-5426. |
| [17] | Larena A;Caceres DA;Vicario C;Fuentes A .Release of a chitosan-hydroxyapatite composite loaded with ibuprofen and acetyl-salicylic acid submitted to different sterilization treatments[J].Applied Surface Science: A Journal Devoted to the Properties of Interfaces in Relation to the Synthesis and Behaviour of Materials,2004(1/4):518-522. |
| [18] | 孔桦,孟洁,郭小天,杨子彬,许海燕.载药纳米羟基磷灰石材料的体外药物缓释研究[J].透析与人工器官,2005(03):12-15. |
| [19] | 肖永红.临床抗生素学[M].重庆:重庆出版社,2004:50-51. |
| [20] | Chen Xiang;Liu Jing;Gao Song et al.[J].Chinese Journal of Biotechnology,2008,24:401-408. |
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