采用快速原型技术构造了3种不同形貌的高分子多孔模板,然后通过浆料灌注和凝胶注模原位凝固技术制备出以β-磷酸三钙为主要成分的三维多孔支架,实现了对骨组织工程支架孔结构的控制。采用微计算机断层扫描技术,在不破坏样品的情况下,精确地对支架的孔隙结构进行了观察和表征,并对支架的物相组成、显微形貌以及抗压强度进行了分析和测试。采用高分子模板制备的规则孔结构支架,大孔隙相互连通构成三维连通结构,并且具有大孔-微孔多级孔结构,抗压强度可达2MPa以上,大孔孔隙率为50%以上。结果表明,通过快速原型技术制备的连通多孔高分子模板,结合原位凝固成型技术,可以制备出孔径和结构可控、孔隙完全连通的β-磷酸三钙支架。
Tissue engineering scaffolds for bone repair have high requirement of interconnectivity and pore geometry.Controllable preparation of pore structure was the key to work out this problem.The objective of this work was to prepare three-dimensional porous inorganic scaffolds with controlled pore structure.The β-tricalcium phosphate slurry with high solid content was poured into the polymer porous moulds with different pore structure,which were fabricated by rapid prototyping technique,then the β-tricalcium phosphate three-dimensional porous scaffolds were obtained by in situ consolidation of gel-casting.The pore structure of scaffolds was characterized by micro-computed tomography(micro-CT),and the phase composition,morphology images and compressive strength of the porous scaffolds were tested.The scaffolds using polymer porous models have connective macro-pores structures.The compressive strength and macro-pores porosity of scaffolds were more than 2MPa and more than 50%.The results indicate this method is an effective way to control the pore structure and pore size of β-tricalcium phosphate tissue engineering scaffold with full interconnectivity.
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