材料期刊网

Calcium-phosphate materials, such as β- TCP could be degraded in vivo , and in the cultured cells in vitro. However, it is still unknown how the materials changed the activity of the cells. In order to understand the mechanism of β- TCP treatment on osteoblasts , cell proliferation rate measurement and microscopic observation were performed during co-culture of rat osteoblasts and β-TCP biomaterials. It was observed that low concentrations of β-TCP-treated cell proliferation rate was a little less than control by MTT assay, while higher concentrations of β- TCP-treated cell proliferation rate was more than control. The proliferation rate of osteoblast trented by β-TCP with bioglass had a similar effect with β-TCP-treated cell. Neither calcium chloride nor sodium monophosphate treatment could stimulate the cell proliferation at the concentration from 0.25 mM to 5 mM. During the treatment of β-TCP , particles could be found in the cells. It suggested that β-TCP could stimulate cell proliferation rate of rat osteoblast with or without bioglass, while Ca2 + and PO43- ( provided by calcium chloride and sodium monophosphate , separately), the degraded products of β- TCP had no significant effect on osteoblast cell growth in a wide range. The stimulating effect of β- TCP on osteoblast cell proliferation might relate to the encapsulation of the material particles into the cells.