暴露于空气中的钛表面容易受到碳氢化合物的污染,本研究的目的是探讨不同程度的碳氢化合物污染对钛片表面生物活性及对成骨细胞黏附和增殖的影响.将酸蚀处理的钛片分成4组,用常规的保存方法分别储存不同的时间.通过扫描电镜,表面轮廓仪,X射线光电子能谱和接触角测量等手段分析钛片表面理化性能的变化,然后在钛片表面进行细胞培养试验.结果表明,钛片的表面形态不会随着储存时间的改变而改变,但是,钛片表面的化学组成随着储存时间的增加而改变,碳含量从25.99%增加到48.16%.另外,碳氢化合物污染还改变了钛片表面润湿性,从最初的亲水性变成了疏水性.细胞培养结果表明,碳氢化合物的污染不利于成骨细胞的黏附与增殖.因此,改良钛表面的保存方法以保持其表面活性是以后研究的方向.
参考文献
[1] | Buser D;Schenk R K;Steinemann S et al.[J].Journal of Biomedical Materials Research,1991,25:889. |
[2] | Kieswetter K;Schwartz Z;Dean D D et al.[J].Critical Reviews in Oral Biology and Medicine,1996,7:329. |
[3] | An YH;Alvi FI;Kang Q;Laberge M;Drews MJ;Zhang J;Matthews MA;Arciola CR .Effects of sterilization on implant mechanical property and biocompatibility.[J].The international journal of artificial organs,2005(11):1126-1137. |
[4] | Zhao L;Mei S;Wang W;Chu PK;Wu Z;Zhang Y .The role of sterilization in the cytocompatibility of titania nanotubes.[J].Biomaterials,2010(8):2055-2063. |
[5] | Park J H;Olivares-Navarrete R;Baier R E et al.[J].Acta Biomaterialia,2011,7(06):2751. |
[6] | Massaro C;Rotolo P;De Riccardis F et al.[J].Journal of Materials Science:Materials in Medicine,2002,13:535. |
[7] | Kasemo B;Lausmaa J .[J].Journal of Biomedical Materials Research,1988,22(A2):145. |
[8] | Klauber C;Lenz L J;Henry P J .[J].International Journal of Oral & Maxillofacial Implants,1990,5:264. |
[9] | Aparicio C;Olive J .[J].International Journal of Oral & Maxillofacial Implants,1992,7:94. |
[10] | Deligianni DD;Katsala N;Ladas S;Sotiropoulou D;Amedee J;Missirlis YF .Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption.[J].Biomaterials,2001(11):1241-1251. |
[11] | Anselme K. .Osteoblast adhesion on biomaterials [Review][J].Biomaterials,2000(7):667-681. |
[12] | Mata A;Su X;Fleischrnan A J et al.[J].IEEE Transactions on NanoBioscience,2003,2:287. |
[13] | Benya P D;Shaffer J D .[J].Cell,1982,30:215. |
[14] | Subbiahdoss G;Kuijer R;Grijpma DW .Microbial biofilm growth vs. tissue integration: "the race for the surface" experimentally studied.[J].Acta biomaterialia,2009(5):1399-1404. |
[15] | Faghihi S;Azari F;Zhilyaev AP;Szpunar JA;Vali H;Tabrizian M .Cellular and molecular interactions between MC3T3-E1 pre-osteoblasts and nanostructured titanium produced by high-pressure torsion.[J].Biomaterials,2007(27):3887-3895. |
[16] | Hallab NJ;Bundy KJ;O'Connor K;Moses RL;Jacobs JJ .Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion.[J].Tissue engineering,2001(1):55-71. |
[17] | van Kooten TG.;Spijker HT.;Busscher HJ. .Plasma-treated polystyrene surfaces: model surfaces for studying cell-biomaterial interactions[J].Biomaterials,2004(10):1735-1747. |
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